摘要
目的了解妊娠期、哺乳期至成年前饮水氟铝联合暴露对子代大鼠骨组织中mi RNA-133a及血清骨特异性碱性磷酸酶(B-ALP)水平的影响。方法将36只健康清洁级SD妊娠大鼠随机分为9组,分别为对照(自来水)组和氟化钠(60、240 mg/L)、氯化铝(600、1 000 mg/L)染毒组以及氟化钠+氯化铝染毒组,每组4只。采用自由饮水方式染毒,母鼠染毒时间为妊娠第0天至仔鼠出生后第21天(断乳);断乳后,从每组随机选取8只仔鼠(雌雄各半),继续以同组浓度和方式染毒至出生后第90天。测定尿、骨中氟、铝的水平及血清B-ALP水平以及骨组织中mi RNA-133a的表达水平。结果与对照组比较,除单独氯化铝染毒组和60 mg/L Na F+1 000 mg/L氯化铝染毒组外,其余染毒组仔鼠的尿氟浓度均升高(P<0.05),氟铝联合染毒对不同性别仔鼠尿氟浓度的影响在各浓度Na F+氯化铝联合染毒组中均表现为拮抗作用(P<0.05);除单独Na F染毒组和240 mg/L Na F+600 mg/L氯化铝染毒组外,其余染毒组仔鼠的尿铝浓度均升高(P<0.05),氟铝联合染毒对尿铝浓度的影响在60 mg/L Na F+1 000 mg/L氯化铝染毒组表现为协同作用,在其余各浓度Na F+氯化铝联合染毒组中均表现为拮抗作用(P<0.05);除单独氯化铝染毒组和60 mg/L Na F+1 000 mg/L氯化铝染毒组外,其余染毒组仔鼠骨氟含量均升高(P<0.05),氟铝联合染毒对不同性别仔鼠骨氟含量的影响在各浓度Na F+氯化铝联合染毒组中均表现为拮抗作用(P<0.05);单独氯化铝染毒组和240 mg/L Na F+1 000 mg/L氯化铝染毒组仔鼠的骨铝含量均升高,氟铝联合染毒对骨铝含量的影响在各浓度Na F+氯化铝联合染毒组中未见交互作用(P>0.05)。与对照组比较,1 000 mg/L氯化铝染毒组和60 mg/L Na F+1 000 mg/L氯化铝染毒组、240 mg/L Na F+1 000 mg/L氯化铝染毒组仔鼠血清中B-ALP的水平均降低(P<0.05),氟铝联合染毒对血清中B-ALP水平的交互作用以拮抗为主(P<0.05);60 mg/L Na F染毒组、600 mg/L氯化铝染毒组和60 mg/L Na F+600 mg/L氯化铝染毒组、60 mg/L Na F+1 000 mg/L氯化铝染毒组、240 mg/L Na F+600 mg/L氯化铝染毒组仔鼠骨组织中mi R-133a的表达水平均升高(P<0.05),氟铝联合染毒对骨组织中mi R-133a表达水平的交互作用以拮抗为主(P<0.05)。结论氟铝联合暴露可能通过升高骨组织中mi R-133a表达和降低血清中B-ALP水平,从而抑制妊娠期、哺乳期至子代大鼠成年前仔鼠骨组织中成骨细胞的活性。
Objective To know the effects of combined exposure of fluorine and aluminum through drinking water during gestation and lactation periods and before adulthood on the expression of mi RNA-133 a in bone tissue and bone-specific alkaline phosphatase（B-ALP） in serum of offspring rats. Methods Thirty-six pregnant SD rats were randomly divided into nine groups, four in each, control group（drinking tap water）,fluoride group（drinking water containing sodium fluoride of 60 mg/L and 240 mg/L）,aluminum group（drinking water containing aluminum chloride of 600 mg/L and 1 000 mg/L）,fluoride＋aluminum joint exposure group. The pregnant rats were treated with fluoride or aluminum through drinking water from pregnant day 0 to postnatal day 21（PND21） of the offspring rats. Eight offspring rats were randomly selected from each group and were treated as above.The levels of fluoride and aluminum in urine and bone were determined. The content of serum ALP was determined by ELISA. The expression of mi R-133 a was measured by real-time fluorescence quantitative PCR. Results Compared with the control group,except the aluminum chloride alone group and 60 mg/L Na F＋1 000 mg/L aluminum chloride group, the fluoride concentrations in urine were higher（P〈0.05）, combined effects of fluoride and aluminum in different gender of the offspring rats on the urine fluoride was antagonism interaction（P〈0.05）; Compared with the control group,except the Na F alone group and 240 mg/L Na F ＋600 mg/L aluminum chloride group, the aluminum concentrations in urine were higher（P〈0.05）, except the synergism of the combined effects of fluoride and aluminum on the urine aluminum in 60 mg/L Na F＋1 000 mg/L aluminum chloride group（P〈0.05）; Compared with the control group,except the aluminum chloride alone group and 60 mg/L Na F＋1 000 mg/L aluminum chloride group, the fluoride concentrations in bone were higher（P〈0.05）, combined effects of fluoride and aluminum in different gender of the offspring rats on the fluorine concentrations in bone was antagonism interaction（P〈0.05）; Compared with the control group,the aluminum concentrations in bone in the aluminum chloride alone group and240 mg/L Na F＋1 000 mg/L aluminum chloride group were higher（P〈0.05）, combined effects of fluoride and aluminum on the aluminum concentrations in bone in those treated groups were no interaction（P〈0.05）. Compared with the control group,the serum B-ALP levels in 1 000 mg/L aluminum chloride group, 60 mg/L Na F＋1 000 mg/L aluminum chloride group and 240 mg/L Na F＋1 000 mg/L aluminum chloride group were decreased（P〈0.05）. Compared with the control group,the expression levels of mi R-133 a in the bone in 60 mg/L Na F group, 600 mg/L aluminum chloride group, 60 mg/L Na F＋600 mg/L aluminum chloride group, 60 mg/L Na F ＋1 000 mg/L aluminum chloride group and 240 mg/L Na F ＋600 mg/L aluminum chloride group were increased（P〈0.05）. Combined effects of fluoride and aluminum on B-ALP and mi R-133 a expression levels were antagonism interaction（P〈0.05）. Conclusion Joint exposure of fluoride and aluminum in the pregnancy and lactation can reduce the BALP level in serum and increase the levels of mi R-133 a in bone, which may be involved in osteoblast activity inhibition in rats offspring.
作者
葛启迪
陈小美
王柱红
张华
谭瑛
罗雨
谢春
GE Qi-di;CHEN Xiao-mei;WANG Zhu-hong;ZHANG Hua;TAN Ying;LUO Yu;XIE Chun(Department of Environmental Health, School of Public Health, Guizhou Medical University, Guiyang, Guizhou 550025, Chin)
出处
《环境与健康杂志》
CAS
北大核心
2018年第2期108-113,共6页
Journal of Environment and Health
基金
国家自然科学基金(81560519)
