摘要
以盆栽一年生‘丰后’梅自根苗、山杏为砧木的‘丰后’梅嫁接苗以及山杏自根苗为试材,设置土壤NaCl含量分别为0(CK)、0.3%、0.6%、0.9%、1.2%和1.5%的盐胁迫处理,观测3种植物盐害情况及其叶片细胞膜透性、抗氧化酶活性、有机渗透调节物质含量的变化,并应用隶属函数值法对各盐浓度处理下3种材料叶片的生理指标进行综合评定,以明确它们的耐盐能力,为梅花向盐渍土分布较广的北方地区推广应用,以及梅花繁殖方法及梅花砧木的选择提供理论依据。结果表明:(1)随着土壤中NaCl含量的递增,‘丰后’梅自根苗、‘丰后’梅嫁接苗及山杏的盐害指数和盐害率不断增大,三者盐害指数在50%时土壤NaCl含量分别是0.545%、0.695%和0.705%,表明3种材料耐盐性由强到弱依次为山杏>‘丰后’梅嫁接苗>‘丰后’梅自根苗。(2)随着盐胁迫时间的延长,3种植物苗木叶片相对电导率(RC)和丙二醛(MDA)含量整体呈先上升后下降的趋势;‘丰后’梅自根苗过氧化物酶(POD)活性总体呈上升趋势,‘丰后’梅嫁接苗和山杏总体均呈现上升趋势,但个别浓度呈先上升后下降趋势;‘丰后’梅自根苗可溶性糖(SS)含量整体呈现上升趋势,‘丰后’梅嫁接苗和山杏均总体呈先下降后上升趋势,但个别较高浓度呈先上升后下降趋势。(3)隶属函数值法对盐抗性的综合评定结果为:山杏>‘丰后’梅嫁接苗>‘丰后’梅自根苗,综合评定结果与3种材料的盐害指数分析结果一致。研究发现,3种植物叶片所受到盐伤害的程度随NaCl胁迫浓度提高而加深,但植株叶片能对中低盐浓度处理产生一定的适应性;以耐盐性较强的山杏作为嫁接砧木后,‘丰后’梅嫁接苗比其自根苗的耐盐性增强,证明嫁接对梅花耐盐性具有一定的促进作用。
One-years-old seedling of Prunus mume ‘Fenghou’,grafted seedling of P.mume ‘Fenghou’and Prunus sibirica treated by NaCl were studied to explain their resistance to salt stress.The soil salt contents were set as 0（CK）,0.3%,0.6%,0.9%,1.2% and 1.5% respectively.Salt injury index,membrane permeability,antioxidant enzyme activity and osmotic adjustment substance contents were detected.and the physiological indexes of three plants leaves were evaluated synthetically by membership function law to determine their salt tolerance ability and to provide the theory basis for the popularization and application in the north,the choice of breeding methods,and the choice of stock selection of P. mume.The results showed as follows：（1）with the increase of soil NaCl content,salt injury index and salt injury rate of seedling of P.mume‘Fenghou’,grafted seedling of P.mume‘Fenghou’and P.sibirica in-creased,which NaCl contents are 0.545%,0.695% and 0.705% when their salt injury index is 50%.It
indicates that the resistance of three plants to NaCl ranked from P.sibirica,grafted seedling of P.mume‘Fenghou’to seedling of P.mume ‘Fenghou’.（2）With the stress days lengthened of soil NaCl,relative conductivity and MDA contents increased firstly and then decreased;POD activity increased but part of grafted seedling of P.mume ‘Fenghou’and P.sibirica increased firstly and then decreased;Soluble sug-ar content of seedling of P.mume ‘Fenghou’increased,Soluble sugar content of grafted seedling of P.mume ‘Fenghou’and P.sibirica decreased firstly and then increased but part of them increased firstly and then decreased.（3）We synthetically assess the resistance of three plants to NaCl ranked from P. sibirica,grafted seedling of P.mume ‘Fenghou’to seedling of P.mume ‘Fenghou’by membership function law,which is the same as the result of salt injury index analysis.The study found that the injured degree of three plants leaves deepened with the salt stress concentration elevated,but they can produce certain adaptability to the lower and medium salt stress.The salt resistance of seedling of P.mume ‘Feng hou’is more stronger than grafted seedling of P.mume ‘Fenghou’when it was treated.The strong salt tolerance of P.sibirica as root stock,explains that grafting can improve the salt tolerance of P.mume.
作者
杨佳鑫
遆羽静
罗燕杰
赵靓
李庆卫
YANG Jiaxin;TI Yujing;LUO Yanjie;ZHAO Liang;LI Qingwei(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation ＆ Molecular Breeding,National Engineering Research Center for Floriculture,Beijing Laboratory of Urban and Rural Ecological Environment,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2018年第4期723-732,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
科学研究与研究生培养共建科研项目-北京实验室-北京城乡节约型绿地营建技术与功能型植物材料高效繁育(2016GJ-03)
关键词
梅花
山杏
嫁接
NACL胁迫
盐害率
生理指标
Prunusmume
Prunussibirica
grafting
NaClstress
saltinjuryindexes
physiologicalindexes
