摘要
目的探讨基质金属蛋白酶3启动子基因5A/6A多态性与老年冠状动脉粥样硬化性心脏病的相关性。方法前瞻性选取2015年10月至2017年10月老年冠状动脉粥样硬化性心脏病患者187例,同期体检健康老年人190例。两组对象血清基质金属蛋白酶3(MMP-3)水平采用酶联免疫吸附测定(ELISA)。两组对象的基质金属蛋白酶3启动子基因5A/6A多态性采用聚合酶链反应(PCR)-限制性片段长度多态性(RFLP)方法测定。对比两组患者的一般资料、MMP-3水平、MMP-3的各基因型和等位基因。结果观察组糖尿病、高血压病史以及吸烟的患者比率明显高于对照组,差异具有显著性(P<0.05)。两组高血压、糖尿病、吸烟史患者基因型及等位基因频率差异均无显著性(P>0.05)。观察组MMP-3水平高于对照组(P<0.05);观察组组内6A/6A MMP-3水平低于基因型5A/5A+5A/6A(P<0.05);观察组MMP-3基因型5A/6A、5A/5A+5A/6A频率,等位基因5A频率明显高于对照组,差异具有显著性(P<0.05)。结论基质金属蛋白酶3启动子区5A/6A多态性与老年冠状动脉粥样硬化性心脏病存在相关性。
Objective To discuss the correlation of Matrix metalloproteinases 3 promoter gene 5 A/6 A polymorphism and senile coronary atherosclerosis heart disease. Methods From October 2015 to October 2017 187 cases of elderly patients with coronary atherosclerosis heart disease and 190 cases of health elderly were selected in this study. Serum levels of matrix metalloproteinase 3 was detected by enzyme-linked immunosorbent assay（ ELISA）,polymerase chain reaction（ PCR）,restriction fragment length polymorphism（ RFLP） method were performed for determining the matrix metalloproteinase 3 promoter gene 5 A/6 A polymorphism. Results There were more diabetes,high blood pressure and smoking patients in observation group with significant difference（ P〈0. 05）,there was no difference in hypertension,diabetes,smoking history of genotype and allele frequency（ P〈0. 05）. The MMP-3 level of observation group was higher than that of the control group（ P〈0. 05）. 6 A/6 A MMP-3 levels in observation group was below genotype 5 A/5 A ＋ 5 A/6 A（ P〈0. 05）. The frequency of MMP-3 genotypes 5 A/6 A,5 A/5 A ＋5 A/6 A in the observation group was significantly higher than that of the control group（ P〈0. 05）. Conclusion There is correlation of Matrix metalloproteinases 3 promoter area 5 A/6 A polymorphism with senile coronary atherosclerosis heart disease.
作者
陆蕙
胡东南
王蓉
彭伟
王婷婷
LU Hui;HU Dong- nan;WANG Rong(Department of Internal Medicine, Beijing Aerospace General Hospital, Beijing 100039, China.)
出处
《临床和实验医学杂志》
2018年第11期1200-1203,共4页
Journal of Clinical and Experimental Medicine
