摘要
以台杨3号茎段和叶片为外植体材料,建立离体再生体系。结果表明:茎段在0.15%HgCl_2溶液中处理10min,污染率为23.3%,出芽率达75.3%,在MS+BA 0.3mg·L^(-1)+NAA 0.1mg·L^(-1)培养基中诱导不定芽的效果较好,平均分化率77.8%;叶片分化培养基为MS+BA 0.5mg·L^(-1)+NAA 0.2mg·L^(-1)+KT 0.2mg·L^(-1)+Ad 40mg·L^(-1),分化率可达87.7%;MS+BA 0.5mg·L^(-1)+KT 0.3mg·L^(-1)+NAA 0.2mg·L^(-1)培养基增殖效果较好;1/2MS+NAA 0.2mg·L^(-1)+CCC 0.5mg·L^(-1)为生根、壮苗培养基,生根率100%。
A regeneration protocol was established using the stems and leaves of fast-growing Taiwan Poplar 3 as explants.The selected disinfection time for the stems was 10 min in a 0.15% HgCl2 solution to result in an infection rate of 23.3% and a budding rate of 75.3%.The optimized medium for generating adventitious buds was MS＋BA0.3 mg· L^-1＋ NAA 0.1 mg· L^-1 to reach an average differentiation rate of 77.88%;that for the leaf differentiation,MS＋BA 0.5 mg·L^-1＋NAA 0.2 mg·L^-1＋KT 0.2 mg·L^-1＋adenine 40 mg·L^-1 with a differentiation rate of 87.7%;that for the proliferation,MS＋BA 0.5 mg·L^-1＋KT 0.3 mg·L^-1＋NAA 0.2 mg·L^-1;and that for rooting,1/2 MS＋NAA 0.2 mg·L^-1＋CCC 0.5 mg·L^-1 with a 100% rooting rate.
作者
黄枝英
庄卫东
汤红玲
马晓娟
刘靓
HUANG Zhi ying;ZHUANG Wei dong;TANG Hong ling;MA Xiao juan;LIU Liang(Quanzhou Agricultural Research Institute, Quanzhou, Fujian 362212, China)
出处
《福建农业学报》
CAS
北大核心
2018年第4期386-390,共5页
Fujian Journal of Agricultural Sciences
基金
泉州市科技计划重点项目(2011N6)
关键词
台湾速生杨树3号
组织培养
植株再生
Fast growing Taiwan Poplar 3
tissue culture
regeneration system
