摘要
[目的]发掘抗旱相关基因,明晰其编码蛋白的理化特征,为糜子育种提供基因元件。[方法]利用生物信息学软件、数据库和在线程序,对糜子Ty1-copia型反转录转座子编码蛋白进行生物信息学分析,同时对该基因进行qRTPCR表达特性分析。[结果]Ty1-copia基因编码蛋白分子式为C483H758N122O135S7,分子量为10 658.49Da,呈弱碱性,是不稳定的疏水蛋白;该蛋白为非分泌型蛋白、无跨膜结构,既不存在于叶绿体上也不存在于线粒体上;二级结构包括4个α螺旋,4个β折叠;三级结构包括1个螺旋结构和2个折叠结构;含4个保守结构域;系统进化树和多重序列比对结果显示糜子保守性较低,与水稻类和毛竹的同源性为64%。实时定量PCR检测结果表明,Ty1-copia表达水平在干旱胁迫处理不同时间点存在显著差异,3h时表达量最高,为对照的2.02倍;8h时该基因呈下调表达,表达量低于对照。[结论]Ty1-copia基因可以由干旱胁迫诱导。
[Objective]Exploring genes related to drought-resistance and clarifing the physical and chemical charateristics of their encoded proteins,genetic elements were provieded for the breeding of common millet.[Methods]Several bioinformatics software,database,and online programs were employed,and bioinformatics analysis of Ty1-copiagene coding protein was conducted.The expression of Ty1-copia gene was assessed by qRT-PCR.[Results]Results showed that the molecular structure of Tyl-copia is C483 H758 N122 O135 S7 with the molecular weight at 10 658.49 Da.It is an unstable and weakly alkaline protein without transmembrane structure.It is a non-secretory protein which couldn＇t be observed on either chloroplast or mitochondria.Fourα-helixs and fourβ-folds were detected in the secondary structure,and a helical structure and two folding structures were found in tertiary structure which also includes four conserved domains.The phylogenetic tree and multiple sequence alignment studies revealed that millet is less conservative,and its homology with rice and bamboo is up to 64%.qRT-PCR result showed that there was a significant difference in expression level of Ty1-copia at different time points under drought stress condition.The expression level of Ty1-copia was the highest at 2.0 hpost treatment,which was 2.02 times higher than that of the control.At 8 h,the gene wasdown-regulated and the expression level was lower than the control.[Conclusion]These bioinformatics analyzes allow us to understand the function,characteristics,gene family and homology of the WRKY gene and its encoded protein.The function of WRKY transcription factor can be further deduced by the function of proso milet homologues.At the same time,it also proved that the gene can be induced in response to drought stress.
作者
何杰丽
薛延桃
王瑞云
陈国荣
陈凌
王海岗
杨美红
乔治军
He Jieli;Xue Yantao;Wang Ruiyun;Chen Guorong;Chen Ling;Wang Haigang;Yang Meihong;Qiao Zhijun(College of Arts and Sciences,Shanxi Agricultural University,Taigu 030801,China;College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Institute of Crop Germplasms Resources of Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2018年第6期22-28,共7页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金项目(31271791)
山西省回国留学人员科研资助项目(2016-066)
山西省自然科学基金(201601D011074)
现代农业产业技术体系建设专项资金(CARS-06-13.5-A16)
山西省重点研发计划(一般项目)(农业)项目(201603D221003-5)
