摘要
为了评价采油井场石油污染的微生物修复潜力,选取油井旁持续污染区(W)、井场曾经污染区(S)、原泥浆池区(M)和周边农田区(F)这4个不同位置,对0~400 cm不同深度土壤进行调查取样.利用高通量测序技术,对样品16S rRNA基因V4区序列进行测定,结合石油含量、三氮等物化性质,分析了微生物的生物多样性、群落组成及其和环境因子的关系.结果表明,石油污染减少了土壤的生物多样性,石油含量、盐度是造成微生物群落结构差异的最主要环境因子.通过组间差异分析发现,W和M土壤不仅存在着大量的石油降解细菌,其中的高盐样品中还含有嗜盐石油降解古菌.S与F具有相似的群落结构,表明石油烃的自然衰减.因此,该井场具有丰富的石油修复菌种资源,具有很好的微生物修复潜力.
To design a reasonable and effective remediation scheme for soil in contaminated sites,it is necessary to understand the microbial communities in the soil. Samples were collected at different depths（ 0 cm to 400 cm） in four locations： one that was persistently contaminated and near an oil well,one that was historically contaminated in the middle of the site,one in a mud pit,and one in farmland. High-throughput sequencing of the V4 region of 16 S rRNA in these samples was performed. In addition to physicochemical properties of the soil,the α-diversity,species composition,and differences in species between groups of microorganisms were analyzed,and a principal coordinate analysis and canonical correlation analysis were conducted. Results showed that oil and salt contents in soils were the dominant factors affecting microbial community structure. Hydrocarbon-degrading microorganisms were abundant in oil-contaminated soils,whereas halophilic hydrocarbon-degrading microorganisms were present in soils with high salt contents. Therefore,hydrocarbon-degrading microorganisms might be useful in remediation of oil-contaminated sites.
作者
蔡萍萍
宁卓
何泽
张敏
石建省
CAI Ping-ping;NING Zhuo;HE Ze;ZHANG Min;SHI Jian-sheng(Institute of Hydrogeology and Enviro 2. School of Resources and Enviromental Geological Sciences, China University nmental Geology Engineering, He 3f Geosciences Chinese Academy of Geological Sciences, Shijiazhuang 050061, China;University of Technology, Hefei 230009, China;Chinese Academy of （Beijing） , Beijing Remediation of Hebei Province, Shijiazhuang 050061, China;5. Key Labora Survey, Shijiazhuang 050061, China） 100083, Chin;4. Key Laboratory of Groundwater tory of Groundwater Remediation of China Geologica)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2018年第7期3329-3338,共10页
Environmental Science
基金
国家自然科学基金项目(41402233)
中国地质科学院水文地质环境地质研究所基本科研业务费专项(SK201702)
关键词
采油井场
石油污染
群落结构
石油降解菌
修复潜力
oil exploitation site
oil contamination
microbial communities
hydrocarbon degradation microorganism
remediationpotential
