摘要
目的:检测miR-34a-5p对人角质形成细胞株HaCaT细胞体外增殖及其靶基因Notch1 mRNA表达的影响。方法:HaCaT细胞体外培养,优化转染浓度,分为4组进行转染:miR-34a-5p模拟物组(mimic组),阴性对照组(NC组),miR-34a-5p抑制物组(inhibitor组),抑制物阴性对照组(inhibitor NC组)。MTT检测细胞增殖情况;RT-q PCR检测Notch1的mRNA表达情况。结果:转染后48小时mimic组、inhibitor组细胞增殖活力分别为39.7%和15.7%,与NC组(21.3%)相比,差异均有统计学意义(均P<0.05)。HaCaT细胞转染后,mimic组和inhibitor组Notch1表达量分别为2.78±1.30和0.37±0.46,与NC组(1.0±0.06)比较,差异均有统计学意义(均P<0.05)。结论:miR-34a-5p促进HaCaT细胞体外增殖,机制可能与Notch1 mRNA表达上调有关。
Objective： To determine the effects of miR-34a-5p on proliferation of HaCaT human keratinocytes and expressions of Notch1 mRNA. Methods： HaCaT cells were cultured in vitro and transfection concentration were optimized. And than,the cells were divided into 4 groups： miR-34a-5p mimic group（ mimic）,negative control group（ NC）,miR-34a-5p inhibitor group（ inhibitor） and inhibitor negative control group（ inhibitor NC）. Cell proliferation was detected by MTT assay. The expression of Notch1 mRNA was detected by RT-q PCR. Results： After transfected for 48 hours,the proliferation vitality of HaCaT cells and the level of Notch1 in NC group were 21. 3% and 1. 0 ± 0. 06 respectively,those in mimic group were 39. 7% and 2. 78 ±1.30,those in inhibitor group was 15.7% and 0.37± 0.46. There were significant differences in the proliferation vitality of HaCaT cells and the level of Notch1 between mimic group,inhibitor group and NC group（ P〈0.05）. Conclusion： miR-34a-5p can promote the proliferation of HaCaT cells,which may be related to the upregulation of Notch1 mRNA.
作者
朱杰
王慧琴
赵宗峰
张慧
康晓静
吴卫东
ZHU Jie;WANG Huiqin;ZHAO Zongfeng;ZHAGN Hui;KANG Xiaojing;WU Weidong(People＇s Hospital of Xinfiang Uygur Autonomous Region, the Center of Clinical Research, Urumqi 830001, China;People＇s Hospital of Xinjiang Uygur Autonomous Region, Department of Dermatology and Venereology , Urumqi 830001, China)
出处
《中国麻风皮肤病杂志》
2018年第6期324-326,共3页
China Journal of Leprosy and Skin Diseases
基金
新疆维吾尔自治区自然科学基金(编号:201521C201)
