摘要
目的:初步观察毫针、火针两种针具的瘤周围刺对荷乳腺癌小鼠肿瘤生长状态、机体免疫功能、预后情况的影响。方法:将对数增长期的4T1乳腺癌细胞株接种于小鼠前肢腋窝后方,建立BALB/c小鼠4T1乳腺癌移植瘤模型,随机分为模型组(18只,不干预)、毫针组(18只,瘤周围刺,留针20min)、火针组(18只,瘤周围刺),同时将正常饲养的小鼠作为空白组(9只)。以7d为1个治疗周期,每个周期的第1、4天干预,每个治疗周期结束后分别取材(肿瘤、胸腺、脾脏、血液),测量并计算对小鼠抑瘤率、胸腺指数、脾脏指数、中性粒细胞与淋巴细胞绝对值比值(NLR)、血小板与淋巴细胞绝对值比值(PLR),共取材3次。结果:抑瘤率方面,火针组各时间点分别是6.043%,26.421%,51.132%,高于毫针组的1.999%,15.367%,45.929%。小鼠免疫方面,火针组各时间点胸腺指数分别是(3.38±0.41),(3.58±0.19),(3.23±0.24),较毫针组同期更加稳定(4.17±0.46),(2.97±0.43),(3.00±0.64)。火针组各时间点脾脏指数分别是(4.76±0.58),(5.78±0.24),(5.29±0.30),较毫针组同期更加稳定(5.10±0.47),(5.69±0.27),(5.11±0.14)。预后方面火针组各时间点PLR、NLR与毫针组同期数据无统计学意义。结论:瘤周围刺对4T1小鼠乳腺癌移植瘤增长具有明显抑制作用,火针优于毫针,且随干预时间延长抑瘤效果越明显,但其预后不如毫针围刺效果佳。
Objective：To preliminarily observe the effects of surrounded acupuncture with filiform needles and fire needles on the tumor growth status,immune function and the prognosis of mice with breast cancer.Methods：Inject the 4 T1 breast cancer cell strain under the skin of the forelimb armpit of the mice.Upon successful model establishment,the mice was randomly divided into 3 groups,which was model group（18,without any intervention）,filiform needling group（18,surrounded acupuncture with filiform for 20 minutes）and the fire needling group（18,surrounded acupuncture with fire needles）.Meanwhile,the general feeding mice were assigned as the normal group（9）.The treatment circle lasted for 7 days,filiform intervention were implemented on the 1 st and 4 th day of the treatment circle.Observe the inhibition rate,measure spleen index and thymus index,detect the neutrophil-lymphocyte ratio（NLR）and platelet-lymphocyte ratio（PLR）at each end of the treatment circle,3 times in total.Results：In terms of inhibition ratio of tumor volume fire needling group was 6.043%,26.421%,51.132% after each treatment period,exceeding the filiform needling group（1.999%,15.367%,45.929% respectively）.In terms of the immunization：thymus index for fire needling group（3.38±0.41,3.58±0.19,3.23±0.24,respectively）was more stable than the filiform needling group（4.17±0.46,2.97±0.43,3.00±0.64）.So as spleen index（fire needling group4.76±0.58,5.78±0.24,5.29±0.30,filiform needling group（5.10±0.47,5.69±0.27,5.11±0.14）.In terms of prognosis：there was no statistical significance between groups in NLR（P〉0.05）,but fire needling group shows statistical significance in PLR（P〈0.05）.Conclusion：Surrounded acupuncture can inhibit the growth of the breast cancer on 4 T1 transplanted tumor mice.The effect of fire needling surrounded acupuncture is more significant than the filiform one.The better effects are shown as the treatment circle is prolonged,but the prognosis is basically the same bwtween two intervention group.
作者
李洪峥
姜欣
刘苗苗
钱旭
黄金昶
Li Hongzheng;Jiang Xin;Liu Miaomiao(School of Acupuncture Moxibustron and Tuina, Beijing University of Chinese Medicine,Beijing 100029)
出处
《陕西中医》
2018年第6期683-686,共4页
Shaanxi Journal of Traditional Chinese Medicine
基金
国家级大学生创新创业课题(201610026034)
关键词
乳腺肿瘤
针刺疗法
动物实验
小鼠
Breast neoplasms Acupuncture therapy Animal experimentation Mice
