摘要
为了鉴定多孔木霉色素的化学结构,并对其合成基因簇进行分析。本研究分离纯化环孢菌素A合成菌多孔木霉(Tolypocladium inflatum)培养液中的色素,然后通过光谱学分析初步鉴定色素的结构。在此基础上,采用生物信息学方法对色素合成基因簇进行解析。多孔木霉色素为蒽醌类物质,分子式为C15H10O5,含有3个苯羟基和2个酮基。T.inflatum色素与镰刀菌红素、孢子黄色素的合成基因簇同源性高,合成T.inflatum色素的基因簇共有43个开放阅读框(ORFs),核心基因为聚酮合酶(polyketide synthase,PKS)合成酶基因。本研究从色素结构和合成基因簇分析入手,为进一步阐明色素的生物合成途径奠定基础。
To identify the chemical structure of pigment from Tolypocladium inflatum,we separated and analyzed the pigment by high pressure preparation liquid chromatography and spectroscopy analysis.Furthermore,we used online bioinformatic analysis software to predict the gene cluster and its function.The results showed that the pigment was an anthraquinone compound with chemical formula of C15 H10 O5,and had three phenoxyl radicals and two ketone groups. The related gene clusters of pigment contain 43 open reading frame（ ORFs） and belong to polyketide synthase（ PKS） type cluster. Our studies can provide a basis for further exploring the biosynthetic pathway of pigment.
作者
洪虹
岳雪
李大成
王炳全
董惠钧
HONG Hong;YUE Xue;LI Dacheng;WANG Bingquan;DONG Huijun(Urology Department of Liaocheng People.s Hospital,Liaocheng 252000,China;School of Pharmacy,Liaocheng University,Liaocheng 252000,China;Biopharmaceutical Institute,Liaocheng University,Liaocheng 252000,China)
出处
《生物加工过程》
CAS
2018年第3期22-28,共7页
Chinese Journal of Bioprocess Engineering
基金
山东省自然科学基金(ZR2015CL001)
山东省抗体制药协同创新中心开放课题(CIC-AD1818)
关键词
多孔木霉
色素
聚酮合酶
合成基因簇
环孢菌素A
生物医药
Tolypocladium inflatum
pigment
polyketide synthase
biosynthetic gene cluster
cyclosporine A
biological medicine
