摘要
目的探讨不同检查方法对乳腺癌侵犯乳头乳晕复合体(NAC)临床检出率的影响。方法收集240例女性原发性乳腺癌患者病理标本,分为观察组与对照组,每组各120例。对照组于乳头的正中矢状面取材,取材深度5 mm,观察组取乳头与乳晕皮肤以及连同皮下10 mm的整块组织,采用自创5刀法取材;两组均分别经常规苏木精-伊红染色(HE)及免疫组化染色(IHC)[细胞角蛋白19(CK-19)为目标蛋白]处理后镜检。结果观察组经HE及IHC处理后的检出率分别为8.33%与24.17%,两者比较差异有统计学意义(P<0.05);对照组经HE及IHC处理后的检出率分别为6.67%与8.33%,两者比较差异无统计学意义(P>0.05);经HE处理后,两组患者检出率比较,差异无统计学意义(P>0.05);经IHC处理后,观察组检出率高于对照组,差异有统计学意义(P<0.05);样本经IHC处理可观察到NAC的微侵犯灶。结论采用乳头矢状面5刀法取材,联合以CK-19为目标蛋白的IHC检查可提高乳腺癌侵犯NAC检出率,更准确地反应乳腺癌NAC受累的真实情况。
Objective To investigate the effect of different examination methods on the clinical detection rate of nipple-areolar complex（NAC） invasion in breast cancer. Methods A total of 240 pathological specimens of primary breast cancer were divided into observation group and control group, with 120 cases in each group. The specimens were taken from the median sagittal plane in the control group for depth of 5 mm, and shose in the observation group were taken from the nipple and areola skin and subcutaneous tissue together with the whole 10 mm by homemade 5 blade. The two groups were treated with regular HES and IHC（CK-19 as target protein） and microscopically examined after the treatment. Results The detection rates in the observation group by HES and IHC were respectively 8.33% and 24.17%, and the detection rate by IHC was significantly higher than that by HES（χ^2 = 9.052, P = 0.008）. The detection rates in the control group by HES and IHC were 6.67% and 8.33%, and the detection rate by IHC was not statistically significantly higher than that by HES（χ^2 = 1.076, P = 0.087）. After HES, the detection rate was not significantly different between the two groups（χ^2 = 0.240, P = 0.103）. After IHC, the detection rate in the observation group was significantly higher than that of control group（χ^2= 11.375, P = 0.002）. NAC micro invasive lesions can be observed in sample treated by IHC. Conclusion Papillary sagittal 5 blade combined with IHC examination with CK-19 can improve detection rate of NAC invasion in breast cancer, which can more accurately reflect the involvement of NAC in breast cancer.
作者
董良鹏
张景航
Liang-peng Dong;Jing-hang Zhang(The First Afliated Hospital of Xinxiang Medical University,Xinxiang, Henan 453100, China)
出处
《中国现代医学杂志》
CAS
2018年第15期110-113,共4页
China Journal of Modern Medicine
