摘要
为了了解改良施肥方式对土壤微生物群落结构的影响,以白菜为试材,采用Illumina MiSeq高通量测序技术,对常规施用化肥和改良施肥(化肥减量、增施有机肥、增施土壤调理剂)条件下白菜根际真菌和细菌群落结构进行了研究。结果表明:改良施肥后增加了白菜产量,缓解了土壤酸化的情况,土壤养分增加,改良施肥方式白菜根际真菌和细菌丰度和多样性大于常规施肥处理,2种施肥方式微生物群落组成和优势类群有较大差异。改良施肥后白菜根际微生物种类增多,优势类群发生了较大变化。
In order to understand the effect of the modified fertilization mode on soil microbiome community structure,Chinese cabbage was collected as the research object,and Illumina MiSeq high throughput sequencing technology was used to analyze the rhizospheric fungal and bacterial community structure of Chinese cabbage under the condition of conventional fertilization and modified fertilization（fertilizer reduction,increasing organic fertilizer and soil conditioner）.The results showed that the improved fertilization increased the yield of cabbage,alleviated soil acidification,and soil nutrient increased.There were significant differences between the microbial community and dominant groups of the two kinds of fertilization methods.The modified fertilization method led the abundance of rhizosphere microbiome increased,the community structure changed,and the dominant fungi groups varied.
作者
陈诚
张小川
蔡小扬
王剑
伏荣桃
卢代华
CHEN Cheng;ZHANG Xiaochuan;CAI Xiaoyang;WANG Jian;FU Rongtao;LU Daihua(Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 6100663;Chengdu Tianjie Organic Agriculture Development Co. Ltd. , Chengdu, Sichuan 610091)
出处
《北方园艺》
CAS
北大核心
2018年第13期108-113,共6页
Northern Horticulture
基金
成都市产业升级牵引工程资助项目(2015-NY01-00119-NC)
关键词
白菜
改良施肥
MiSeq高通量测序
微生物群落
Chinese cabbage
modified fertilization
MiSeq high-throughput sequencing
microbial community
