摘要
目的探究绞股蓝总皂苷(Gyp)对口腔鳞状细胞癌细胞系SCC9增殖和凋亡的影响,并初步探讨相关机制。方法用不同浓度Gyp(70、85、100μg/ml)培养SCC9细胞系,未用Gyp处理作为对照组(Ctrl组),MTT法检测细胞增殖;流式细胞术检测细胞凋亡;荧光定量实时PCR和蛋白免疫印迹检测细胞中Notch1和Jagged1 mRNA和蛋白表达。结果 Gyp浓度分别为70、85、100μg/ml时,SCC细胞增殖率分别为62.15±8.13、50.08±7.14、24.83±5.63,均显著低于Ctrl组(P<0.05);细胞凋亡率分别为23.54±2.17、37.01±3.62、60.03±7.85,均显著高于Ctrl组(P<0.05);Notch1 mRNA表达分别为2.24±0.17、2.46±0.23、2.97±0.31,蛋白表达分别为0.98±0.08、1.26±0.09、1.71±0.13,均显著高于Ctrl组(P<0.05);Jagged1 mRNA表达分别为0.94±0.11、0.51±0.09、0.26±0.06,蛋白表达分别为1.43±0.15、0.73±0.08、0.24±0.06,均显著低于Ctrl组(P<0.05);SCC9细胞荧光强度分别为65.69±11.32、113.12±15.94、163.32±19.48,均显著高于Ctrl组(P<0.05)。结论 Gyp以剂量依赖的方式抑制OSCC细胞系SCC9的增殖并诱导其凋亡,其中Notch信号通路活化在这一过程中起重要作用。
Objective To investigate the effect of gypenosides （Gyp） on proliferation and apoptosis of oral squamous cell carcinoma cell line SCC9 and to explore its underlying mechanism. Methods SCC9 cells were cultured with different concentrations of Gyp （70, 85 and 100 μg/ml）, the untreated with Gyp as a control group （Ctrl group）. Cell prolifera- tion was detected by MTr assay. Apoptosis was detected by flow cytometry. The mRNA and protein expression of Notchl and Jaggedl were detected by real-time PCR and Western blot. Results The proliferative rates of SCC were 62.15 ± 8.13, 50.08 ±7.14 and 24.83 ±5.63 at Gyp concentrations of 70, 85 and 100 izg/ml, respectively, which were signifi- cantly lower than those in Ctrl group （ P 〈 0.05 ） ; the apoptotic rates were 23.54 ±2.17, 37.01 3.62 and 60.03 ± 7.85, respectively, which were significantly higher than that of the Ctrl group （ P 〈 0.05 ） ; Notchl mRNA expression was 2.24 ±0.17, 2.46 ± 0.23, 2.97 ± 0.31 ; the protein expression was 0.98 ± 0.08, 1.26 ± 0.09 and 1.71 ±0.13, respectively, which were significantly higher than that of the Ctrl group （ P 〈 0.05 ） ; Jaggedl mRNA expression were 0.94 ±0.11, 0.51 ±0.09, 0.26 ±0.06; the protein expressions were 1.43 ±0. 15, 0.73 ±0.08 and 0.24 ±0.06, re- spectively, which were significantly lower than those in the Ctrl group （ P 〈 0.05 ） ; the fluorescence intensity of SCC9 cells were 65.69 ±11.32, 113.12 ± 15.94 and 163.32± 19.48 respectively, which were significantly higher than that of the Ctrl group （ P 〈 0.05 ）. Conclusion Gyp can inhibit the proliferation and induces apoptosis of OSCC cell line SCC9 in a dose-dependent manner. Notch signaling pathway activation plays an important role in this process.
作者
王梁
吴伟
陈龙
潘凌峰
李幼华
徐明
汪柳静
蔡雅卫
WANG Liang;WU Wei;CHEN Long(Department of Stomatology, the Second Hospital of Ningbo, Ningbo, Zhejiang 315010, China)
出处
《中华全科医学》
2018年第9期1441-1445,共5页
Chinese Journal of General Practice
基金
浙江省中医药科技计划项目(2016ZA175)
浙江省宁波市自然科学基金(2016A610147)
