摘要
【目的】探讨白介素-27（IL-27）联合腺苷脱氨酶（ADA）检测对结核性胸膜炎的诊断价值。【方法】选择2016年5月至2017年10月本院收治的90例因胸腔积液住院患者，其中结核性胸腔炎46例（观察组），其他性质胸腔积液44例（对照组）。所有研究对象均于入院后行胸膜腔穿刺术，采集胸腔积液，分离胸腔积液上清液；均于入院清晨空腹采集外周静脉血，分离血清。采用比色法测定ADA含量，采用酶联免疫吸附法（ELISA法）测定IL-27含量。比较两组胸腔积液、血清IL-27、ADA水平变化，IL-27和ADA联合诊断效能，及IL-27与ADA相关性。【结果】观察组胸膛积液、血清IL-27、ADA含量均显著高于对照组，差异均具有统计学意义（P〈0．05）。IL-27联合ADA诊断灵敏度、特异度和准确度高于IL-27与ADA单项；结核性胸膜炎患者血清IL-27与ADA呈线性正相关。【结论】IL-27联合ADA检测可提高结核性胸膜炎诊断效能，可作为辅助诊断结核性胸膜炎有效指标。
[Objective] To investigate the diagnostic value of interleukin -27 （IL-27） combi ned with aden- osine deaminase （ADA） in the diagnosis of tuberculous pleurisy.[Methods] Ninety cases of pleural effusion hospitalized in our hospital from May 2016 to October 2017 were selected, including 46 tuberculous pleural ef- fusion patients （observation group） and 44 patients with other pleural effusion （control group）. All of the sub- jects were performed pleural puncture after admission, collecting pleural effusion, separating pleural effusion supernatant, collecting peripheral venous blood at the early morning of admission, and separating serum. The content of ADA was measured by colorimetric method and the content of IL-27 was determined by enzyme linked immunosorbent assay （ELISA）. The operation was determined strictly according to the specification standard of the kit. The changes of IL-27 and ADA content of pleural effusion, the changes of serum IL-27 and ADA, the combination of IL-27 and ADA, and the correlation between IL-27 and ADA were compared be- tween the two groups. [Results]Pleural effusion IL-27 and ADA content of the observation group were higher than those of the control group （ P 〈0.05） ; the serum levels of IL-27 and ADA were higher than those of the control group （ P 〈0.05）; ADA IL-27 diagnostic sensitivity, specificity and accuracy were higher than those of IL-27 and ADA single; tuberculous pleural effusion and IL-27 ADA showed a linear positive correlation. [Conclusion]IL-27 combined with ADA detection can improve the diagnostic efficiency of tuberculous pleurisy and can be used as an effective indicator for the diagnosis of tuberculous pleurisy.
作者
张艳丽
武丽
王涛
李福建
ZHANG Yan-li;WU Li;WANG Tao;et al(Tuberculosis Department, the Infectious Disease Hospital of Baoding, Hebei Province, Chin)
出处
《医学临床研究》
CAS
2018年第6期1107-1109,共3页
Journal of Clinical Research
关键词
结核
胸膜
白细胞介素类
腺苷脱氨酶
Tuberculosis
Pleural
Interleukins
Adenosine Deaminase
