摘要
目的探讨基质金属蛋白酶-9(matrix metalloproteinases-9,M M P9)、富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)在原发性肝癌病情和预后评估中的价值.方法选取2012-04/2014-04在宁海县中医医院进行手术的54例原发性肝癌患者作为研究组,其中25例癌旁肝组织作为对照组,利用免疫组织化学染色法检测两组研究对象中MMP9、SPARC的表达,分析原发性肝癌组织和癌旁肝组织中MMP9、SPARC表达的差异,并分析其表达与临床病理特征和预后的关系.结果 M M P9、SPARC在肝癌组织中表达的强阳性率和评分均显著高于癌旁肝组织(P<0.05).MMP9的表达在原发性肝癌组织不同T分期、N分期、AJCC分期和分化程度的表达中具有统计学差异(P<0.05).SPARC的表达在原发性肝癌组织不同T分期、N分期、AJCC分期和肿瘤分化程度的表达中具有统计学差异(P<0.05).单因素分析结果显示,MMP9、SPARC强阳性表达及T分期、N分期、AJCC分期均是影响肝癌患者总生存期(overall survival,OS)和无病生存期(disease-free survival,DFS)的独立预后因素(P<0.05).多因素分析结果显示,MMP9、SPARC强阳性表达是肝癌患者OS和DFS的预后因素(P<0.05).结论 MMP9、SPARC在肝癌组织中表达明显较高,且在不同T分期、N分期、AJCC分期和肿瘤分化程度的肝癌组织中具有差异.MMP9、SPARC强阳性表达是影响肝癌患者OS和DFS的预后因素,可能为肝癌严重程度和预后评估提供帮助.
AIM To analyze the value of matrix metalloproteinase-9（MMP9） and secreted protein acidic and rich in cysteine（SPARC） in evaluating disease condition and prognosis of primary liver cancer. METHODS Fifty-four patients with primary liver cancer who underwent surgery at Ninghai Traditional Chinese Medicine Hospital from April 2012 to April 2014 were selected as a study group, and 25 tumor adjacent liver tissues were selected as a control group. The expression of MMP9 and SPARC in liver cancer and tumor adjacent liver tissues was detected by immunohistochemical staining. The difference in MMP9 and SPARC expression between primary liver cancer tissues and tumor adjacent liver tissues was analyzed, and the relationship between MMP9 and SPARC expression and clinicopathologic features and prognosis was also explored. RESULTS The strong positive rates and scores of MMP9 and SPARC expression in liver cancer tissues were significantly higher than those in tumor adjacent liver tissues（P〈0.05）. MMP9 and SPARC expression was significantly correlated with T stage, N stage, AJCC stage, and tumor differentiation in liver cancer tissues（P〈0.05）. Univariate analysis showed that strong MMP9 and SPARC expression, T stage, N stage, and AJCC stage were independent risk factors for overall survival（OS） and disease-free survival（DFS） in patients with liver cancer（P〈0.05）. Multifactor analysis showed that strong MMP9 and SPARC expression were risk factors for OS and DFS in patients with liver cancer（P〈0.05）. CONCLUSION The expression of MMP9 and SPARC in primary liver cancer tissues is significantly high, and is significantly correlated with T stage, N stage, AJCC stage, and tumor differentiation. Strong MMP9 and SPARC expression are risk factors for OS and DFS in liver cancer, which may provide help for the evaluation of disease condition and prognosis of primary liver cancer.
作者
杨建村
Jian-Chun Yang(Department of Clinical Laboratory, Ninghai Traditional Chinese Medicine Hospital, Ninghai 315600, Zhejiang Province, China)
出处
《世界华人消化杂志》
CAS
2018年第17期1036-1043,共8页
World Chinese Journal of Digestology
