摘要
目的:观察苦参素(OM)对人肝癌细胞株HepG2细胞增殖、细胞内ATP含量、上清液乳酸生成速率的影响,探讨其可能的机制。方法:体外培养人肝癌细胞株HepG2,CCK-8法观察不同浓度、不同作用时间的OM对HepG2细胞增殖的影响;高效液相色谱技术、乳酸试剂盒分别检测苦参素作用48 h后细胞内ATP、上清液乳酸乳酸生成速率影响。结果:OM可以抑制人肝癌细胞株HepG2细胞增殖,其作用效果随药物浓度、作用时间增加而增强(P<0.05);不同浓度苦参素作用48 h后细胞内ATP含量、上清液乳酸生成速率显著下降(P<0.05)。结论:OM可以抑制人肝癌细胞株HepG2细胞增殖,呈现时间-剂量依赖性,作用机制可能是抑制细胞有氧糖酵解使细胞内ATP含量下降有关。
Objective：To observe the effects of oxymatrine（OM） on the proliferation、The level of intracellular ATP and Lactic acid concentration in the culture supernatant of HepG2 hepatoma cell,and to disscuss potential mechanism.Method：HepG2 hepatoma cell was cultured in vitro,CCK-8 method was used to detect the proliferation effect at the different concentrations and different times;a high performance liquid chromatography and enzymatic colorimetric method were used to measure the levels of intracellular ATP and the rate of lactic acid concentration growth in the culture supernatant after treating with 48 h oxymatrine respectively.Result：There was significant difference in inhibited effect of OM on the proliferation of HepG2 hepatoma cell,and with the increase of OM concentration and prolonging the time of action, the inhibition effect of OM on the human hepatoma cell line HepG2 was more obvious（P〈0.05）.The level of intracellular ATP lower and the rate of Lactic acid concentration growth in the culture supernatant After treating with 48 h OM by the different concentrations（P〈0.05）.Conclusion：OM can inhibit the proliferation of HepG2 hepatoma cell,and there is time and dose depended.The mechanism of OM anti-hepatoma may be that through targeting of the glycolytic pathway and results in decreased ATP.
作者
蒋旗
黄赞松
苏建伟
曹聪
JIANG Qi;HUANG Zansong;SU Jianwei(Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Chin)
出处
《中国医学创新》
CAS
2018年第13期27-30,共4页
Medical Innovation of China
基金
广西壮族自治区卫生和计划生育委员会自筹经费项目(Z2016418)
广西高校桂西地区微生物感染研究重点实验室项目(Kfkt2016018)
