摘要
目的：观察消癌解毒方对荷瘤小鼠瘤组织内IL-6/STAT3信号通路的影响。方法：将40只雄性CT26荷瘤小鼠随机分为模型对照组（生理盐水灌胃2 m L·d^-1）,消癌解毒方低、中、高剂量组（消癌解毒方中药灌胃5,10,20 g生药·kg^（-1））,奥沙利铂组（奥沙利铂腹腔注射5 mg·kg^-1）,每组8只。连续给药14 d,末次给药2 h后处死小鼠,剥取瘤组织。采用末端转移酶标记技术（TUNEL）法观察小鼠瘤组织中细胞凋亡率;免疫组化染色法检测小鼠瘤组织中Cleaved Caspase-3蛋白表达;蛋白质免疫印迹（Western blot）法检测小鼠瘤组织中信号转导和转录激活因子3（signal transducers and activators of transcription 3,STAT3）,磷酸化的信号转导和转录激活因子3（phospho-STAT3,p-STAT3）,白细胞介素-6（interleukin-6,IL-6）蛋白的表达。结果：TUNEL结果显示,与模型对照组比较,消癌解毒方各组小鼠瘤组织中细胞凋亡指数明显升高（P〈0.01）;免疫组化结果显示,与模型对照组比较,消癌解毒方中、高剂量组可以显著增加Cleaved Caspase-3蛋白表达（P〈0.05）;Western blot结果表明与模型对照组比较,消癌解毒方中、高剂量组可以显著降低pSTAT3,IL-6的蛋白表达（P〈0.05）。结论：消癌解毒方可以促进肿瘤细胞凋亡,其机制可能与抑制IL-6/STAT3信号通路,上调Cleaved Caspase-3的表达有关。
Objective： To investigate the effect of Xiao＇ai Jiedu Prescription on IL-6/STAT3 signal pathway in CT26 tumor-bearing mice. Methods： Forty male CT26 tumor-bearing mice were divided randomly into model control group（ i. g. NS 2 m L·d^-1）,Xiao＇ai Jiedu Decoction low,medium and high dose groups（ ig Xiao＇ai Jiedu Prescription 5,10,15 g·kg^-1）,oxaliplatin group（ i. p. oxaliplatin 5 mg·kg^-1） with 8 mice in each group. Continuous administration was lasted for 14 days. After the final administration for 2 hours,mice were sacrificed and tumor tissues were stript. TUNEL assays was adopted to detect the apoptosisindex（ AI） of tumor tissue. The proteins of Cleaved Caspase-3 in tumor tissue were detected by immunohistochemistry. Western blot was performed to detect the proteins of STAT3,p-STAT3 and IL-6 in tumor tissue. Results： Tunel results showed that compared with model control group,each Xiao＇ai Jiedu Decoction group,s AI was high（ P〈0. 01）. The immunohistochemical staining results showed that the protein of Cleaved Caspase-3 were increased（ P〈0. 05） in medium and high dose Xiao＇ai Jiedu Decoction groups. And the Western blot results showed that Xiao＇ai Jiedu Prescription decreased the expressions of p-STAT3 and IL-6 protein compared with the model control group（ P〈0. 05）. Conclusion： Xiao＇ ai Jiedu Prescription can improve the apoptosis in tumor tissue. The mechanism may be correlated with inhibition of IL-6/STAT3 signal pathway and up-regulation of the expression of Cleaved Caspase-3.
作者
石文静
谭佳妮
沈卫星
徐长亮
孙东东
程海波
SHI Wenjing;TAN Jiani;SHEN Weixing;XU Changliang;SUN Dongdong;CHENG Haibo(Translational Medicine Research Center of Nanjing University of Chinese Medicine,Key Laboratory of Famous Doctors＇ Proved Recipe Evaluation and Transformation of State Administration of Traditional Chinese Medicine,Provincial Lab of Anticarcinoma Proved Recipe Research and Engineering Industrialization,Nanjing 210023 ,Jiangsu, China;The First Clinical Medical College of Nanjing University of TCM,Nanjing 210023,Jiangsu,China;TCM Treatment on Tumor Collaborative Innovation Center of Jiangsu Provine,Nanjing 210023 ,Jiangsu,China)
出处
《辽宁中医杂志》
CAS
2018年第6期1289-1292,I0005,共5页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81373511,81573910,81673559,81403079)
江苏省自然科学基金(BK20141467,BK20161045)
江苏省高校自然科学基金面上项目(16KJB360001)
江苏高校优势学科建设工程二期资助项目
