摘要
以贵州瓮福磷尾矿土壤为原料,从中分离纯化得到具有较高解磷能力的细菌。利用溶磷圈试验筛选出具有明显溶磷圈的细菌,通过形态学特征、生理生化试验、16S rDNA基因序列分析及系统发育树对其进行初步鉴定。再以磷酸三钙为唯一磷源对筛选所得菌株进行液态培养,探索不同菌株解磷能力与培养液pH值之间的关系,并通过钼锑抗比色法测定3株细菌的最大解磷能力。从分离纯化得到的4株细菌筛选出具有明显溶磷圈的细菌PSB1、PSB3和PSB4,初步鉴定菌株PSB1为普城沙雷氏菌(Serratia plymuthica),PSB3为嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia),PSB4为泡囊短波单胞菌(Brevundimonas vesicularis),最大解磷能力分别为148.87μg/mL、153.84μg/mL和146.76μg/mL。与国内已报道的文献相比,实验筛选所得的3株细菌都是具有较高解磷能力的菌种。菌株PSB1和PSB4培养液中pH值与其解磷能力存在显著的负相关性,而菌株PSB3不存在,其解磷机理还需进一步研究。
High phosphate-solubilizing bacteria（PSBs）were isolated from phosphate tailings soil in Wengfu Group,Guizhou Province.PSBs were screened by dissolving phosphate ring test. And the genera of the phosphate-solubilizing bacteria were primarily determined by a combination of morphological characteristics identification,physiological and biochemical test,16S rDNA gene sequences analysis,and phylogenic trees. The maximum phosphate-solubilizing activity of these strains were determined by using Mo-Sb colorimetry with tricalcium phosphate as the only phosphate source,and the relationships between the abilities of different PSBs and the pH value of the culture medium were explored. The PSB1,PSB3 and PSB4 of 4 PSBs isolated from phosphate tailings soil presented obvious phosphate-solubilizing rings.The PSB1,PSB3,and PSB4 were identified to be Serratia plymuthica,Stenotrophomonas maltophilia,and Brevundimonas vesicularis,respectively. The maximum phosphate-solubilizing activities of these strains were 148.87 μg/mL,153.84 μg/mL,and 146.76 μg/mL,respectively. Compared with information in the published articles in China,the three strains all had high phosphate-solubilizing ability. For the PSB1 and PSB4,there were a significantly negative correlation between the phosphate-solubilizing ability and the pH of the culture medium,but not so for the PSB3,and the phosphate-solubilizing mechanism of the PSB3 should be further investigated.
作者
陈佳兴
秦琴
邱树毅
王雪郦
CHEN Jia-xing;QIN Qin;QIU Shu-yi;WANGXue-li(Guizhou Provincial Key Laboratory of Fermentation Engineering and Biological Pharmacy, Guizhou University, Guiyang 550025;School of Liquor and Food Engineering, Guizhou University, Guiyang 550025;College of Life Sciences, Guizhou University, Guiyang 550025)
出处
《生物技术通报》
CAS
CSCD
北大核心
2018年第6期183-189,共7页
Biotechnology Bulletin
基金
贵州省省校合作计划项目(黔科合LH字[2016]7452)
中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2017JJ002)
关键词
磷尾矿土壤
解磷细菌
解磷能力
鉴定
phosphate tailings soil
phosphate-solubilizing bacteria
phosphate-solubilizing ability
identification
