摘要
目的探讨生骨密骨方对骨质疏松(OP)大鼠血清雌二醇(E_2)、胰岛素样生长因子-1(IGF-1)、肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)的影响及可能的作用机制。方法选择6个月龄健康清洁级雌性SD大鼠48只,手术摘除其双侧卵巢,随机分为对照组(A组)、雌二醇组(B组)、中药组(C组),每组16只。B组给予戊酸雌二醇片,C组给予生骨密骨方水煎浓缩液(每1mL含生药1.19g),A组给予等量生理盐水,连续灌服12周。结果建模成功后,B组大鼠子宫指数为(112.85±22.63)mg/20 g,与C组的(114.92±21.55)mg/20 g无显著差异(t=0.320,P=0.751>0.05),B组及C组大鼠子宫指数均高于A组大鼠的(86.45±19.62)mg/20 g(t=7.154,6.592,P<0.01);C组大鼠血清中E_2,IGF-1均显著高于A组(t=7.693,12.853,P<0.01),与B组无明显差异(t=0.296,1.251,P=0.769,0.221>0.05);C组大鼠血清中IL-6,TNF-α均显著低于A组大鼠(t=10.729,11.036,P<0.01),与B组无明显差异(t=0.953,1.569,P=0.348,0.127>0.05)。结论生骨密骨方可通过改善骨代谢生化指标,降低骨转换率,起到防治骨质疏松的作用。
Objective To investigate the effect of Shenggumigu Recipe on the levels of E2,IGF-1,TNF-α and IL-6 in the rats with osteoporosis and its possible mechanism.Methods Totally 48 healthy and clean female SD rats at age of six months were selected and their bilateral ovaries were removed by surgery,the rats were randomly divided into the control group（ group A）,E2 group（ group B） and TCM group（ group C）,16 cases in each group.Group B was given Estradiol Valerate Tablets,group C was given concentrate decoction of Shenggumigu Recipe（ 1 m L containing 1.19g crude drug）,and group A was given irrigation of the same amount of normal saline for 12 weeks.Results After successful modeling,the uterine index of group B was（ 112.85 ± 22.63）mg/20g,which was not significantly different from（ 114.92 ± 21.55） mg/20 g in group C（ t = 0.320, P = 0.751 0.05）.The uterine indexes in group B and group C were higher than（ 86.45 ± 19.62） mg/20 g in group A（ t = 7.154,6.592, P〈0.01）.The levels of E2 and IGF-1 in group C were significantly higher than those in group A（ t = 7.693,12.853, P〈0.01）,but which were not significantly different from those in group B（ t=0.296,1.251, P = 0.769,0.221 0.05）.The levels of serum IL-6 and TNF-α in group C were significantly lower than those in group A（ t=10.729, 11.036, P〈0.01）, but which were not significantly different from those in group B（ t = 0.953, 1.569, P =0.348, 0.127〉0.05）.Conclusion Shenggumigu Recipe can reduce bone turnover by improving the biochemical indexes of bone metabolism,so as to achieve the prevention and treatment of osteoporosis.
作者
常波伦
柴仪
李倩
杨家祥
刘烁炜
Chang Bolun;Chai Yi;Li Qian;Yang Jiaxiangl;Liu Shuowei(Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang,Hebei,China 050011;Hebei Friendship Hospital,Shijiazhuang,Hebei,China 050030)
出处
《中国药业》
CAS
2018年第15期17-19,共3页
China Pharmaceuticals
关键词
生骨密骨方
中药
骨质疏松
细胞因子
大鼠
Shenggumigu Recipe
traditional Chinese medicine
osteoporosis
cytokine
rats
