摘要
为了研究叶下珠多酚纳米脂质体的最佳制备条件,以叶下珠多酚包埋率为优化指标,采用响应面法优化得到叶下珠多酚纳米脂质体的最佳制备条件。利用激光粒度仪测定脂质体的粒度分布,并研究纳米脂质体在pH 1.2和pH 7.4缓冲液中的释放行为和释放机理。结果表明:水化介质用量、胆固醇与卵磷脂质量比、多酚浓度三个因素之间的相互作用对包埋率影响不显著,但各因素都对包埋率影响显著,影响显著性的大小顺序为多酚浓度>胆固醇与卵磷脂质量比>水化介质用量;叶下珠纳米脂质体的最佳制备条件为水化介质用量16m L、胆固醇与卵磷脂质量比1∶2.5、多酚浓度1.7mg/m L,此条件下包埋率为67.21%。叶下珠纳米脂质体的平均粒径为259nm,其释放符合Higuchi和Ritger-peppas模型,为扩散释放机理,在pH7.4缓冲液的释放速度比pH1.2缓冲液的快。
In this study,the optimal preparation conditions of Phyllanthus urinarum polyphenol nanoliposomes were optimized by response surface method with embedding ratio as the optimization index. The particle size distribution of the nanoliposomes was measured by laser particle size analyzer,and the release behavior and release mechanism of the nanoliposomes in p H 1.2 and p H 7.4 buffer solution were studied. The results showed that the interaction effects of hydration medium amount,cholesterol to lecithin mass ratio and polyphenol concentration on the embedding ratio were not significant,but either of them alone had a significant effect on polyphenol embedding ratio. The order of influencing significance was polyphenol concentration,cholesterol to lecithin mass ratio and hydration medium amount. The optimal preparation conditions for the nanoliposomes were 16 m L of hydration medium,1 ∶ 2.5 of cholesterol to lecithin mass ratio,1.7 mg/m L of polyphenol concentration,under which the embedding ratio was 67.21%. The average particle diameter of the liposomes was 259 nm. The release of the liposomes was consistent with the Higuchi and Ritger-peppas models and was a diffusion release mechanism. The release rate in p H7.4 buffer solution was faster than that in p H1.2 solution.
作者
刘永
彭嘉欣
李方冰
叶如霞
陈嘉豪
詹晓玲
郭依敏
LIU Yong;PENG Jia-xin;LI Fang-bing;YE Ru-xia;CHEN Jia-hao;ZHAN Xiao-ling;GUO Yi-min(School of Food and Pharmaceutical Engineering,Zhaoqing University,Zhaoqing 526061)
出处
《中国食品添加剂》
CAS
2018年第6期61-65,共5页
China Food Additives
基金
国家级大学生创新创业训练计划项目(201710580009)
肇庆市科技计划项目(2014F012)
关键词
叶下珠
纳米脂质体
粒径分布
释放行为
Phyllanthus urinaria
nanoliposomes
particle size distribution
release behavior
