摘要
肥胖在细胞水平上主要是脂肪细胞蓄积甘油三酯和前脂肪细胞分化为成熟脂肪细胞的能力决定的。本研究显示,茶褐素RTB(粗TB)、TB1(MW>50ku)、TB2(5ku<MW<50ku)以及TB3(MW<5ku)作用3T3-L1成熟脂肪细胞72 h后,可显著抑制其增殖,而对3T3-L1前脂肪细胞的增殖作用影响不大。通过转录组学分析发现,不同分子质量的茶褐素对3T3-L1成熟脂肪细胞基因差异表达明显。从显著富集通路中筛选出以下差异基因:相对于正常对照组,RTB-100组的DNA聚合酶ε和δ显著上调,核糖核苷二磷酸还原酶α2、RNA聚合酶(β、I、I、III(RPABC5))、线粒体3-甲基巴豆酰Co A羧化酶β、核糖核苷二磷酸激酶7、CTP合成酶、驱动蛋白、CYP450、线粒体BCAAs转氨酶的表达显著下调。TB1-100组的二氢硫辛酸脱氢酶显著上调,线粒体三功能酶(α、β)、胞质乙酰转移酶、线粒体脂酰基Co A脱氢酶、BCAAs转氨酶的表达显著下调。TB2-100组的G-CSF、NF-κB(α、β、p105)、趋化因子(CXC10、14,CC2)显著上调,IRGM、Hbα2、IκBα、糖原磷酸化酶、IL-10、E2F3、VEGF(A、B)、丙酮酸激酶(M1/M2)、低密度脂蛋白受体相关蛋白LRP显著下调。TB3-100组的G-CSF、TN-FSF12、TNFRSF1和TNFRSF 19、趋化因子(CXC14、CC)、IL-1、IL-17、IL-24、IL-2、IL-11显著上调,EGF、VEGF3、VEGF102显著下调。这说明前脂肪细胞分化为成熟脂肪细胞达到80%时,加入不同分子质量的茶褐素(100 mg/L)干预,可引起细胞中相关通路及基因表达的显著变化,且不同分子质量的茶褐素所致基因表达谱差异明显,TB2作用最强烈。
At the cellular level, obesity is mainly determined by the ability of fat cells accumulated triglycerides and the ability of preadipocytes differentiate into mature adipocytes. The study showed that RTB, TB1（MW〉50 ku）, TB2（5 ku〈MW〈50 ku） and TB3（MW〈5 ku） can significantly inhibit the proliferation of 3 T3-L1 adipocytes after treating for72 hours, but the proliferation of 3 T3-L1 preadipocytes had little effect. Further by transcriptome analysis we found that the effect of different molecular weight of TB on the gene expression of 3 T3-L1 adipocytes was significantly different.Compared with the control group, the DNA polymerase ε and δ were significantly up-regulated, and the expressions of rib nucleoside diphosphate reductase α2, polymerase（β,I,I,III（RPABC5））,3-methyl-Croton-Co A carboxylase β,nucleoside diphosphokinase 7, CTP synthase, kinesin, CYP450 and BCAAs transaminase were significantly down-regulated in RTB-100 group. The dihydrolipoic acid dehydrogenase was significantly up-regulated in TB1-100 group, but the expressions of TP（α, β）, acetyl transferase, acyl-Co A dehydrogenase and BCAAs transaminase were significantly down-regulated in TB1-100 group. The G-CSF, NF-κB（α, β, p105）and chemokine（CXC10, 14, CC2）were significantly up-regulated, and the expressions of IRGM, Hbα2, IκBα, glycogen phosphorylase, IL-10, E2 F3, VEGF（A and B）, pyruvate kinase and low density lipoprotein receptor-related protein（LRP） were significantly down-regulated in TB2-100 group. The G-CSF, TNFRSF1, TNFSF12, TNFRSF19, chemokine（CXC14, CC）, IL-1, IL-17, IL-24, IL-2 and IL-11 were significantly up-regulated, and the expressions of EGF,VEGF3,VEGF102 were significantly down-regulated in TB3-100 group. It suggested that TB can cause significant changes in cell-related pathways and gene expression when the differentiation of preadipocytes into mature adipocytes reached 80% and after adding different molecular TB（100 mg/L） intervention. The profiles of gene expression caused by different molecular TB was significantly different, TB2 is the strongest.
作者
刘春
冯励
彭春秀
谭超
龚加顺
Liu Chun;Feng Li;Peng Chunxiu;Tan Chao;Gong Jiashun(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201;College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第6期249-261,共13页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(31260195)
