摘要
目的:探索联苯型新木脂素类化合物厚朴酚葡萄糖苷(magnolol-2-O-β-D-glucopyranoside,Mag-glu)对人乳腺癌细胞增殖、侵袭、迁移能力的影响,并对其可能机制进行研究。方法:MTT法检测化合物对MCF-7和MDA-MB-231细胞增殖的影响;细胞划痕实验和Transwell小室法检测化合物对人乳腺癌细胞运动、侵袭和迁移能力的影响;Western blotting法检测化合物对缺氧诱导因子1α(HIF-1α)、基质金属蛋白酶-9(MMP-9)和环氧化酶2(COX-2)蛋白表达的影响。结果:Mag-glu具有抑制MCF-7和MDA-MB-231细胞增殖的作用,且呈现浓度和时间依赖性。划痕实验结果表明,经过不同浓度的Mag-glu处理MDAMB-231细胞24 h后,细胞水平运动运动能力明显下降(P<0.01)。Transwell结果显示,经过不同浓度的Mag-glu处理细胞24 h和48 h后,细胞迁移和侵袭能力显著下降。Western blotting结果显示,随着Mag-glu浓度的增加,HIF-1α、MMP-9、COX-2的表达均下调。结论:Mag-glu在体外能够抑制人乳腺癌细胞的增殖、侵袭和迁移,其机制可能是抑制了HIF-1α信号途径,导致其下游顾客蛋白COX-2和MMP-9的表达下调有关。
Objective： To investigate the effects of magnolol-2-O-β-D-glucopyranoside（ Mag-glu） on the proliferation,invasion and migration of human breast cancer cells,and its possible mechanism. Methods： The effects of Mag-glu on the proliferation of MCF-7 and MDA-MB-231 cells were detected using MTT assay. The effects of Mag-glu on the migration and invasion ability of human breast cancer cells were analyzed using the wound healing assay and transwell assay,respectively. The effects of Mag-glu on the expression levels of hypoxia-inducible factor 1α（ HIF-1α）,matrix metalloproteinase-9（ MMP-9） and cyclooxygenase-2（ COX-2） were detected using Western blotting. Results： Mag-glu could inhibit the proliferation of MCF-7 and MDA-MB-231 cells in a concentration-and timedependent manner. The migration ability of MDA-MB-231 cells decreased significantly after 24 h of treatment with Mag-glu（ P〈0. 01）.Transwell assay results showed that cell migration and invasion abilities significantly decreased after 24 h and 48 h of treatment with different concentrations of Mag-glu. The expression levels of HIF-1α,MMP-9 and COX-2 down-regulated with the increasing of the concentration of Mag-glu. Conclusions： Mag-glu can inhibit the proliferation,invasion and migration of human breast cancer cells in vitro,the mechanism of which may be inhibiting the HIF-1α signaling pathway to lead to the down-regulated expression of COX-2 and MMP-9.
作者
苗健
马涛
李楠
张松松
霍强
吴成柱
MIAO Jian;MA Tao;LI Nan;ZHANG Song-song;HUO Qiang;WU Cheng-zhu(School of Pharmacy,Bengbu Medical College,Bengbu Anhui 233030,Chin)
出处
《蚌埠医学院学报》
CAS
2018年第5期567-572,共6页
Journal of Bengbu Medical College
基金
国家自然科学基金青年项目(81302671)
关键词
厚朴酚葡萄糖苷
乳腺癌细胞
增殖
侵袭
迁移
magnolol glucoside
breast cancer cell
proliteration
invasion
migration
