摘要
目的:探讨鱼油对KKAy糖尿病小鼠糖代谢及磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)/蛋白激酶B(protein kinase B,Akt)信号通路的影响。方法:40只KKAy小鼠,分为模型组和鱼油低、中、高剂量组,每组10只。另设10只C57BL/6小鼠为正常对照组。灌胃12周后,测定血糖、胰岛素、脂联素、瘦素及PI3K/Akt信号通路中相关基因的表达水平。结果:与模型组相比,鱼油低剂量组血糖水平极显著降低(P<0.01),鱼油低、中剂量组胰岛素水平显著提高(P<0.05),鱼油各剂量组的脂联素水平显著提高(P<0.05)。此外,鱼油各剂量组可上调胰岛素受体底物-1、PI3K、Akt、葡萄糖转运蛋白-4基因的表达,其中低剂量鱼油对Akt和葡萄糖转运蛋白-4基因的调控效果更显著。结论:低剂量鱼油可能通过调节PI3K/Akt信号通路改善糖代谢。
Objective： To investigate the effect of fish oil on glucose metabolism and phosphatidylinositol 3-kinase（PI3 K）/protein kinase B（Akt） signaling pathway in diabetic KKAy mice. Methods： Forty KKAy mice were randomly assigned into four groups： diabetic model group, low-dose fish oil group, medium-dose fish oil group and high-dose fish oil group. Ten male C57 BL/6 mice served as a non-diabetic control group. Blood and tissue samples were collected after 12 weeks of treatment. Blood glucose, serum insulin, adiponectin, leptin, and the m RNA expressions of insulin receptor substrate-1（IRS-1）, PI3 K, Akt and glucose transporter-4（GLUT-4） were detected. Results： Compared with the model group, fasting blood glucose was reduced significantly in the low-dose fish oil group（P〈0.01）, fasting insulin was increased in both the low and medium-dose groups（P〈0.05）, and adiponectin level was significantly increased in all the treatment groups（P〈0.05）. In addition, fish oil at all doses up-regulated the m RNA expression of IRS-1, PI3 K, Akt and GLUT-4 and at low dose had more significant effect on the m RNA expression of Akt and GLUT-4. Conclusion： Fish oil at low dose may improve glucose metabolism by regulating the PI3 K/Akt signaling pathway.
作者
胡名媛
王锋
马永建
孙桂菊
HU Mingyuan;WANG Feng;MA Yongjian;SUN Guiju(Key Laboratory of Environmental Medicine and Engineering,Ministry of Education,Department of Nutrition and Food Hygiene,School of Public Health,Southeast University,Nanjing 210009,China;Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第11期126-131,共6页
Food Science
基金
国家自然科学基金面上项目(81573144)
