摘要
目的建立HPLC法同时测定降糖甲片(地黄、黄芪、太子参等)中梓醇、麦角甾苷、吉奥诺苷B_1、马替诺皂苷、毛蕊异黄酮-7-O-β-D-葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素、太子参环肽B的含有量。方法该药物甲醇提取液的分析采用Agilent TC-C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.1%冰醋酸为流动相,梯度洗脱;体积流量0.9 m L/min;柱温30℃;检测波长203、210、254、330 nm。结果 9种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.86%~100.30%,RSD 0.74%~1.68%。结论该方法稳定可靠,可用于降糖甲片的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B_1,martinoside,calycosin-7-O-β-D-glucoside,ononin,calycosin,formononetin and heterophyllin B in Jiangtangjia Tablets（ Rehmanniae Radix,Astragali Radix,Pseudostellariae Radix,etc.）. METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent TC-C_（18） column（ 4. 6 mm ×250 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 1% glacial acetic acid flowing at 0. 9 m L/min in a gradient elution manner,and the detection wavelengths were set at 203,210,254,330 nm. RESULTS Nine constituents showed good linear relationships within their own ranges（ r 0. 999 0）,whose average recoveries were 96. 86%-100. 30% with the RSDs of 0. 74%-1. 68%. CONCLUSION This stable and reliable method can be used for the quality control of Jiangtangjia Tablets.
作者
梁龙
陈镜楼
刘忠
刘立
宋红萍
LIANG Long;CHEN Jing-lou;LIU Zhong;LIU Li;SONG Hong-ping(Department of Pharmacy,The Fourth Hospital of Wuhan,Wuhan 430034,Chin)
出处
《中成药》
CAS
CSCD
北大核心
2018年第6期1312-1315,共4页
Chinese Traditional Patent Medicine
基金
湖北省卫生计生科研基金资助项目(WJ2017M189)
关键词
降糖甲片
化学成分
HPLC
Jiangtangjia Tablets
chemical constituents
HPLC
