摘要
采用色谱法从广藿香内生真菌Daldinia eschscholtzii发酵液中分离得到9个化合物,通过波谱数据分析分别鉴定为dihydrosphaerolone(1)、2,3-dihydro-5-hydroxy-2-methyl-4H-1-benzopyran-4-one(2)、6-hydroxymellein(3)、4,8-dihydroxy-1-tetralone(4)、8-methoxy-1-naphthol(5)、helicascolide A(6)、1-(2,6-dihydroxyphenyl)butan-1-one(7)、1-(2,6-dihydroxyphenyl)ethan-1-one(8)、diisobutyl phthalate(9),其中化合物5、9为首次从该属真菌中分离得到。细胞毒活性测试显示化合物6、7对肿瘤细胞SF-268、MCF-7、NCI-H460、Hep G-2具有较好的抑制活性,IC50值在16.52~28.42μmol/L之间。
Nine compounds were isolated from the fermentation broth of endophytic fungus Daldinia eschscholtzii from Pogostemon cablin by using various chromatographic methods such as silica gel,reverse silica gel,Sephadex LH-20,preparative TLC and HPLC. Their structures were identified as dihydrosphaerolone（ 1）,2,3-dihydro-5-hydroxy-2-methyl-4 H-1-benzopyran-4-one（ 2）,6-hydroxymellein（ 3）,4,8-dihydroxy-1-tetralone（ 4）,8-methoxy-1-naphthol（ 5）,helicascolide A（ 6）,1-（ 2,6-dihydroxyphenyl）-butan-1-one（ 7）,1-（ 2,6-dihydroxyphenyl） ethan-1-one（ 8）,diisobutyl phthalate（ 9）,respectively,based on spectroscopic analysis. Among them,compounds 5 and 9 were isolated from the genus Daldinia for the first time. Compounds 6 and 7 showed moderate growth-inhibitory activities in vitro against SF-268,MCF-7,NCI-H460 and Hep G-2 tumor cell lines with IC50 values ranging from 16. 52 to 28. 42 μmol/L.
作者
卢梦梦
陈玉婵
刘洪新
李赛妮
李浩华
陶美华
郝再彬
章卫民
LU Meng-meng;CHEN Yu-chan;LIU Hong-xin;LI Sai-ni;LI Hao-hua;TAO Mei-hua;HAO Zai-bin;ZHANG Wei-min(State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070,China;College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541006,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第7期1176-1180,共5页
Natural Product Research and Development
基金
国家自然科学基金(31600271)
国家973前期专项(2014CB460613)
广东省科技计划(2015A030302060,2014A030304050)
广东省自然科学基金(2015A030313710)
