摘要
目的:研究Th17、Treg细胞及其相关细胞因子对溃疡性结肠炎发病的影响。方法:选取2013年6月~2016年7月在同济医院消化内科明确诊断为溃疡性结肠炎患者78例为病例组,选择同期因腹痛、腹泻或体质量减轻在消化内科经胃肠镜等相关检查排除消化道器质性病变的患者52例为对照组,采用流式细胞仪检测2组患者外周血中Th17、Treg细胞的比例,采用ELISA法检测2组患者外周血中IL-17、IL-10的表达水平。结果:病例组患者外周血中Th17细胞比例较对照组升高,Treg细胞比例较对照组降低,同时Th17/Treg比值较对照组升高(P<0.05);随着病情的加重,病例组患者Th17细胞在外周血中的比例逐渐升高,Treg细胞在外周血中的比例逐渐降低,Th17/Treg比值也逐渐升高(P<0.05);IL-17在外周血中的水平逐渐升高(P<0.05),而IL-10在外周血中的水平逐渐降低(P<0.05)。病例组患者外周血中IL-17水平较对照组升高,IL-10水平较对照组降低(P<0.05)。结论:溃疡性结肠炎患者中存在Th17、Treg细胞比例失衡,这可能是溃疡性结肠炎发病的免疫原因之一,维持Th17/Treg细胞的平衡,是防止溃疡性结肠炎发生的途径之一。
Objective： To explore the effects of Th17,Treg cells and related cytokines on the pathogenesis of ulcerative colitis. Methods： Seventy-eight patients with ulcerative colitis,definitely diagnosed in the department of gastroenterology of Tongji Hospital between June 2013 and July 2016,were included in case group,and another 52 patients,undergoing gastroenteric endoscopy of the lesions due to abdominal pain,diarrhea or weight loss,were recruited as controls. Flow cytometry was used to detect the proportion of Th17 and Treg cells,and ELISA was performed to determine the IL-17 and IL-10 levels in peripheral blood from the two groups of patients. Results： Elevated Th17 proportion,decreased Treg cells,and increased Th17/Treg ratio were seen in patients in the case group（ P〈 0. 05）. Th17 cells in peripheral blood as well as Th17/Treg ratio tended to gradually increase,and Treg cells to decrease with aggravation of the disease in the case group（ P〈 0. 05）. Patients in the case group had higher IL-17,yet lower IL-10 level than those in the control group（ P〈 0. 05）,and IL-17 level was gradually increased and IL-10 level was gradually decreased in the case group（ P〈 0. 05）. Conclusion： Disproportionate Th17 and Treg cells were found in patients with ulcerative colitis,suggesting that this imbalance may be one of the possible causes of ulcerative colitis as well as maintenance of the balance of Th17/Treg cells can be one of the ways to prevent occurrence of this disorder.
作者
陈孝国
王荣芋
施培华
CHEN Xiaoguo;WANG Rongyu;SHI Peihua(Department of Internal Medicine,School of Medicine,Tongji University,Shanghai 200092,Chin)
出处
《皖南医学院学报》
CAS
2018年第4期325-327,331,共4页
Journal of Wannan Medical College
