摘要
为了从分子水平上解析野生大豆胰蛋白酶抑制剂的特异性,应用RT-PCR方法从即墨野生大豆(Glycine soja Sieb.et Zucc)中扩增出胰蛋白酶抑制剂两种编码基因Kunitz型(KTI,654 bp)及Bowman-Birk型(BBI,357 bp)的基因全长序列,并对两个基因序列做生物信息学分析。分别登录到Gen Bank Accession No.AB112031.1和Accession No.AB081833.1并进行同源性鉴定,证明这两个基因为丝氨酸蛋白酶抑制剂基因家族中的成员。结果表明:即墨野生大豆的KTI基因与野生大豆(Glycine soja,No.AB308134.1)、大豆(Glycine max,No.EF541136.1)的KTI基因序列同源性都为99%,具有6个保守基序;BBI基因与野生大豆(Glycine soja,No.AB081834.1)、大豆(Glycine max,NM_001250058.3)BBI基因序列同源性均为99%,具有5个保守基序,通过二级、三级结构分析发现与栽培大豆有着明显的差别。
In order to analyze the specificity of wild soybean trypsin inhibitor in order to analyze the the molecular level, the full-length sequence of Kunit type trypsin inhibitor （KTI） including 654 bp and Bowman-Birk trypsin inhibitor （BBI） including 357 bp were amplified from Jimo wild soybean （Glycine soja Sieb. et Zucc） by RT-PCR technology, and bioinformaties analysis was performed on the two genes. The gene were sequenced and identified by GenBank Accession No. ABl12031.1 and GenBank Accession No. AB081833. 1. Homology analysis based on Jimo wild soybean KTI gene and BBI gene showed that both genes belonged to serine protease inhibitor gene family. The results showed that the similarity of KTI gene was 99% a- mong Jimo wild soybean, Glycine soja （No. AB308134. 1 ） and C, lycine max （No. EF541136. 1 ） ,including six conserved motifs. The similarity of BBI gene was 99%, among Jimo wild soybean, Glycine soja （No. AB081834. 1 ） and Glycine max （NM 001250058. 3 ）, including five conserved motifs. The secondary and three-dimensional structure analysis showed significant differences from cultivated soybean.
作者
徐一榕
朱妍
张燕茹
王旻
张莉
XU Yi-rong;ZHU Yan;ZHANG Yan-ru;WANG Min;ZHANG Li(College of Food Sciences,Ocean University of China,Qingdao 266003,Chin)
出处
《大豆科学》
CAS
CSCD
北大核心
2018年第4期517-524,共8页
Soybean Science
基金
山东省自然科学基金(ZR2015CM010)
山东省重点研发计划(2017YYSP013)
关键词
野生大豆
胰蛋白酶抑制剂
基因
生物信息学
Glycine soja Sieb. et Zucc
Trypsin inhibitor
Gene
Bioinformatics
