摘要
目的构建Miller Ⅲ度牙龈退缩动物模型,为探索Miller Ⅲ度牙龈退缩的治疗奠定基础。方法选择2只成年雄性比格犬,每只犬选择4颗犬齿采取去除颊侧软硬组织显露牙根表面的方法来建立Miller Ⅲ度牙龈退缩动物模型,通过检测造模术后1、2、4、6、8周的垂直向牙龈退缩量(vertical gingival retraction,VGR)、水平向牙龈退缩量(horizontal retraction,HGR)、角化龈宽度(keratosis tissue width,KTW)、牙龈组织厚度(gingival tissue thickness,GTT)、探诊深度(probing depth,PD)评估动物模型是否建立成功。结果经过临床指标的观察,PD造模前后均小于3 mm,无深牙周袋形成;造模前VGR为0 mm,造模后VGR的范围为5~6.38 mm,VGR造模前后对比差异有统计学意义(P<0.05),术后VGR结果按时间点分组进行对比,术后2、4、6、8周各组之间差异均无统计意义(P>0.05);造模前HGR为0 mm,造模后HGR在10.5 mm上下波动,HGR造模前后对比差异有统计学意义(P<0.05),对术后HGR结果按时间点分组进行单因素方差分析,组间对比结果差异无统计学意义(F=0.952,P=0.45);造模前KTW范围为6~9 mm,造模后KTW在2 mm上下波动,KTW造模前后对比差异有统计意义(P<0.05),术后KTW结果按时间点分组进行对比,术后各组之间差异均无统计学意义(P>0.05)。造模前GTT为1.5 mm,造模后GTT的范围为1.5~2 mm,术前及术后GTT结果按时间点分组对比,结果显示术后1周与术后2周对比差异无统计学意义(P>0.05),术后1周与其他各组对比差异有统计学意义(P<0.05)。结论本实验所采用的方法成功构建出Miller Ⅲ度牙龈退缩动物模型,并在创口愈合后能够保持稳定。
Objective To construct a Miller class Ⅲ gingival recession animal model and to lay the foundation for exploring the treatment of Miller class Ⅲ gingival recession. Methods Two adult male beagle dogs were selected, and four teeth from each beagle dog were selected to establish an experimental Miller class Ⅱ gingival recession model. The root surface was revealed by removing the soft and hard tissues of the buccal side. The success of the model was determined by measuring the vertical gingival retraction （VGR）, horizontal retraction （HGR）, keratosis tissue width （KTW）, gingival tissue thickness （GTT）, and probing depth （PD） at 1, 2, 4, 6, and 8 weeks after modeling. Results After observing the clinical indexes, the PDs before and after the modeling were all smaller than 3 mm and no deep-period pock-ets were formed. The VGR before modeling was 0 mm, and the VGR range after modeling was 5-6.38 mm. A comparison of the before and after modeling results showed that this difference was statistically signfieant （P 〈 0.05）. The postoperative VGR results were grouped according to fimepoint. A comparison between the two groups showed that the differences at 2, 4, 6 and 8 weeks postoperatively were not statistically signlifieant （P 〉 0.05）. The HGR before the modeling was 0 mm, and the HGR fluctuated around 10.5 mm after the modeling, and this difference was statistically significant （P 〈 0.05）. The HGR results were grouped by timepoint after surgery, and a one-way analysis of showed that the differences between the two groups were not statistically signifieant （P 〉 0.05）. The KTW range before modeling was 6 - 9 mm, and it fluctuated around 2 mm after modeling, and this difference was statistically signifieant （P 〈 0.05）. The KTW results were glouped by timepoint after surgery, and they indicated that significant differences did not occur be- tween the groups postoperatively （P 〉 0.05）. The pre-modeling GTT was 1.5 mm, and the GTT range after modeling was 1.5-2 mm. The preoperative and postoperative GTT resuhs were grouped by timepoint, and the results showed that significant differences did not occur between 1 week and 2 weeks after surgery （P = 0.123）, although a statistically significant difference was observed at 1 week postoperatively between this group and the other groups （P 〈 0.05）. Conclusion The method used in this experiment can successfully build a Miller class Ⅲ gingival recession animal model, and the model remains stable after wound healing.
作者
庞罡
徐燕
王莹
叶兴如
何家林
谢贤哲
蒋鹏
辛保见
PANG Gang;XU Yan;WANG Ying;YE XingTu;HE Jialin;XIE Xianzhe;JIANG Peng;XIN Baojian(Department of Periodontology,Affil- iated Stomatology Hospital of Anhui Medical University,Hefei,230032,China)
出处
《口腔疾病防治》
2018年第8期496-503,共8页
Journal of Prevention and Treatment for Stomatological Diseases
基金
安徽省教育厅自然科学重大项目(KJ2017ZD17)
安徽医科大学安科生物校企合作项目(K2015011)
关键词
牙龈退缩
上皮下结缔组织移植术
动物模型
角化龈宽度
根面覆盖
Gingival recession
Subepithelial connective tissue graft
Animal model
Keratinized tissue width
Root coverage
