摘要
建立了测定茶叶中前花青素的高效液相色谱分析方法。样品经过甲醇提取,采用C18柱(4.6 mm×250 mm)进行分离检测,外标法定量,流动相为水/甲醇/异丙醇/甲酸(73/13/6/8),流速为1.0 m L/min,检测波长为525 nm。前花青素在25.0~400μg/m L范围内呈良好线性,线性相关系数为0.9998,回收率为88.7%~95.4%,相对标准偏差(RSD)为6.7%,检出限为0.3 mg/100 g。实验结果表明,该方法准确可靠,灵敏度较高和重现性较好,适用于茶叶中前花青素的定性和定量检测。
An analytical method for the determination of proanthocyanidins in tea was established by high performance liquid chromatograph. The samples were extracted with methanol and separated on a C18 column（ 4. 6 mm ×250 mm） HPLC separation of proanthocyanidins was quantified by the external standard method. The mobile phase was water/methanol/isopropanol/formic acid（ 73/13/6/8） at a flow rate of 1. 0 m L/min. Detection wavelength was 525 nm.The proanthocyanidins had a good linearity in the range of 25. 0 ~ 400 μg/m L with a correlation coefficient of 0. 9998.The recoveries of samples were in the range of 88. 7% ~ 95. 4% and the relative standard deviations（ RSD） were 6. 7%.The detection limit was 0. 3 mg/100 g. Experimental results showed that the method had accuracy reliability and high sensitivity. It was suitable for the qualitative and quantitative detection of tea leaves.
作者
钟永红
马艳芳
黄子荣
黄锦贵
梁勇
刘敏
梁家佑
肖林平
ZHONG Yong-hong;MA Yan-fang;HUANG Zi-rong;HUANG Jin-gui;LIANG Yong;LIU Min;LIANG Jia-you;XIAO Lin-ping(China National Analytical Center（Guangzhou）,Guangdong Guangzhou 510030,China)
出处
《广州化工》
CAS
2018年第14期85-87,共3页
GuangZhou Chemical Industry
基金
佛山市食品安全检测与评价科技创新平台(No:2014AG10007)
关键词
前花青素
茶叶
高效液相色谱
proanthocyanidines
tea leaves
high performance liquid chromatography
