摘要
目的建立HPLC法同时测定葛兰心宁软胶囊中异黄酮类成分3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、大豆苷元的含量。方法超声提取法制备样品,采用Prevail C18色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈(A)-0.5%甲酸溶液(B),梯度洗脱;流速:1.0 m L·min^(-1);检测波长:250 nm;柱温:25℃。结果 3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、大豆苷元分别在0.006~0.030,0.040~0.200,0.012~0.060,0.001 2~0.006 0,0.001 2~0.006 0 mg·m L^(-1)范围内线性关系良好;平均加样回收率为97.16%~98.50%,RSD均小于1.9%。结论该方法简便、准确,可用于葛兰心宁软胶囊中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、大豆苷元的含量测定,为葛兰心宁软胶囊的质量控制提供参考。
Objective To establish a method for simultaneously determination of 3 ＇-hydroxy puerarin,puerarin,3＇-methoxy puerarin, daidzin and daidzein in Gelanxinning soft capsule. Methods Samples were prepared byultrasonic extraction. HPLC analysis was carried out using a Prevail C18 column（5 μm,250 mm × 4.6 mm i.d.）,with acetonitrile（A）-0.5% formic acid solution（B） as mobile phase by gradient elution,and a flow rate of 1.0 m L·min^（-1）,a detection wavelength at 250 nm and a column temperature of 25 ℃. Results Good linear relationshipswere found for 3 ＇-hydroxy puerarin, puerarin, 3＇-methoxy puerarin, daidzin, and daidzein in the range of0.006-0.030 mg·m L-1,0.040 0-0.200 0 mg·m L-1,0.012 0-0.060 0 mg·m L^（-1）,0.001 2-0.006 0 mg·m L^（-1）,0.001 2-0.006 0 mg·m L^（-1）,respectively. The average recovery rates ranged from 97.2% to 98.5%,and all RSDs were lessthan 1.9%. Conclusion The established method is simple and accurate, and can be used for simultaneousdetermination of 3 ＇-hydroxy puerarin,puerarin,3＇-methoxy puerarin,daidzin and daidzein in Gelanxinning softcapsule. The study also provides reference for the quality control of Gelanxinning soft capsule.
作者
王新雨
李岩
唐浩
汪碧涛
WANG Xinyu, LI Yah, TANG Hao, WANG Bitao(Lingnan Institute of Technology, Guangzhou 510663 Guangdong, Chin)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2018年第4期479-482,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
2017年广东省高职教育医药卫生类教育教学改革课题(2017008)
