摘要
背景:结直肠癌的发病率和死亡率居高不下,早期诊断和治疗能提高整体生存率,血清肿瘤标记物的联合检测有望提高筛查效率。目的:探讨血清胰岛素样生长因子结合蛋白2(IGFBP-2)、白细胞介素8(IL-8)和癌胚抗原(CEA)三种肿瘤标记物联合检测对结直肠癌的诊断价值。方法:采用固相抗体芯片检测16例结直肠癌患者和16名正常人血清中13种肿瘤标记物的表达。以液相悬浮芯片进一步验证81例结直肠癌患者和14名正常人中高表达的肿瘤标记物。采用ROC曲线评价上述指标单项检测和联合检测对结直肠癌的诊断价值。结果:与正常人相比,结直肠癌患者血清IGFBP-2、IL-8和CEA表达显著升高(P<0.05)。这三项指标联合检测对结直肠癌的诊断敏感性为87.7%,特异性为92.9%,AUC为0.933,均高于单指标检测。结论:IGFBP-2、IL-8、CEA联合检测可提高结直肠癌的诊断效率,可作为临床诊断结直肠癌的辅助方法。
Background： Incidence and mortality of colorectal cancer remain high,early diagnosis and treatment can improve the overall survival rate,combined detection of tumor markers can improve the screening rate. Aims： To investigate the diagnostic value of combined detection of insulin-like growth factor binding protein 2（ IGFBP-2）,interleukin（ IL）-8 and carcinoembryonic antigen（ CEA） for colorectal cancer. Methods： Expressions of 13 tumor markers were detected in 16 colorectal cancer patients and 16 controls by antibody microarray. Highly expressed tumor markers were verified in 81 colorectal cancer patients and 14 controls by Luminex liquid microarray. ROC curve was used to evaluate the diagnostic value of single or combined tumor markers for colorectal cancer. Results： Compared with controls,serum contents of IGFBP-2,IL-8 and CEA in colorectal cancer group were significantly increased（ P 〈 0. 05）. The sensitivity,specificity,AUC of combined IGFBP-2,IL-8 and CEA for diagnosing colorectal cancer were 87. 7%,92. 9%,0. 933,respectively,which were better than those of single tumor marker. Conclusions： Combined detection of IGFBP-2,IL-8 and CEA can increase the diagnostic efficiency for colorectal cancer and may provide a useful method for detection of colorectal cancer.
作者
顾静莉
李文娜
曹勤
黄融
马嘉骅
顾伟威
张庆华
范竹萍
宋凯
GU Jingli;LI Wenna;CAO Qin;HUANG Rong;MA Jiahua;GU Weiwei;ZHANG Qinghua;FAN Zhuping;SONG Kai(Health Care Center,Renfi Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai（200127;Shanghai Ministry Key Laboratory of Disease and Health Genomics,Chinese National Human Genome Center at Shanghai,Shanghai;Wayen Biotechnologies（Shanghai）,Inc.,Shanghai)
出处
《胃肠病学》
2018年第7期400-404,共5页
Chinese Journal of Gastroenterology
基金
上海市第四轮公共卫生三年行动计划重点学科建设"健康教育与促进学"(15GWZK1001)
上海市科技发展基金项目(1402H192700
15441906800
1602H195000
1702H1B9800)
