摘要
目的探讨双侧内脏大神经切断对犬急性坏死性胰腺炎(ANP)并发肝损伤的影响。方法选取健康成年杂种犬24只,随机分为假手术组(SO组,n=8)、急性坏死性胰腺炎组(ANP组,n=8)和ANP+内脏大神经切断组(GSNT组,n=8)。分别于术前2 h、术后12 h、24 h、3 d、5 d、7 d抽取外周静脉血,检测血清淀粉酶(AMY)、ALT、AST水平;7 d后处死动物,取胰腺组织、肝脏,肉眼及光镜下观察胰腺及肝脏病理改变并进行评分,Western Blot检测肝组织中p-NF-κB p65蛋白的表达。采用单因素方差分析以及重复测量资料的方差分析进行组间比较,进一步两两比较采用LSD-t检验。结果术前2 h,3组动物的血清AMY、ALT、AST水平之间差异均无统计学意义(P值均>0.05);术后ANP组及GSNT组血清AMY、ALT、AST水平,胰腺及肝脏病理评分,以及肝组织中p-NF-κB p65蛋白表达水平均较SO组显著升高(P值均<0.05);GSNT组血清ALT、AST水平以及胰腺和肝脏病理评分、肝脏p-NF-κB p65蛋白表达水平均较ANP组显著降低(P值均<0.05);术后ANP组与GSNT组血清AMY水平差异无统计学意义(P>0.05)。结论 NF-κB的激活在犬ANP发展过程中起重要作用,切断双侧内脏大神经可减轻犬ANP时肝功能的损伤,该保护作用可能与下调肝脏NF-κB的活性有关。
Objective To investigate the effect of bilateral greater splanchnic nerve transection on hepatic injury in dogs with acute necrotizing pancreatitis（ ANP）. Methods A total of 24 healthy adult mongrel dogs were randomly divided into sham-operation group（ SO group with 8 dogs）,ANP model group（ ANP group with 8 dogs）,and ANP ＋ bilateral greater splanchnic nerve transection group（ GSNT group with 8 dogs）. Peripheral venous blood samples were collected at 2 hours before surgery and at 12 and 24 hours and 3,5,and 7 days after surgery to measure the serum levels of amylase（ AMY）,alanine aminotransferase（ ALT）,and aspartate aminotransferase（ AST）. The dogs were sacrificed at 7 days after surgery,and the pancreatic and hepatic tissues were harvested to observe their pathological changes by visual inspection and under a light microscope. Western blot was used to measure the protein expression of p-NF-κB p65 in liver tissue. A one-way analysis of variance and a repeated-measures analysis of variance were used for comparison between groups,and the least significant difference t-test and wilcoxon rank sum test were used for further comparison between two groups. Results At 2 hours before surgery,there were no significant differences in serum levels of AMY,ALT,and AST between the three groups（ P 〉0. 05）. Compared with the SO group,the ANP group and the GSNT group had significant increases in the serum levels of AMY,ALT,and AST,pancreatic and liver pathological scores,and the protein expression of p-NF-κB p65 in liver tissue after surgery（ all P〈 0. 05）. After surgery,the GSNT group had significantly lower serum levels of ALT and AST,pancreatic and liver pathological scores,and protein expression of p-NF-κB p65 in liver tissue than the ANP group（ all P〈 0. 05）,while there was no significant difference in the serum level of AMY between these two groups（ P〉 0. 05）. Conclusion The activation of NF-κB plays an important role in the progression of ANP in dogs. Bilateral greater splanchnic nerve transection can alleviate liver injury in dogs with ANP,and such a protective effect may be associated with the downregulation of NF-κB activity in the liver.
作者
桂洋
孙君军
杨延辉
刘伟峰
杨天保
褚智杰
杨成
王杜
刘江波
GUI Yang;SUN Junjun;YANG Yanhui(Department of Hepatobiliary Surgery,First Affiliated Hospital,College of Clinical Medicine,Henan University of Science and Technology,Luoyang,Henan 471000,China)
出处
《临床肝胆病杂志》
CAS
北大核心
2018年第8期1733-1739,共7页
Journal of Clinical Hepatology
基金
国家自然科学基金资助项目(U1504815)
河南科技大学创新基金(4032-13350049)
关键词
胰腺炎
急性坏死性
肝损伤
NF-ΚB
内脏神经
pancreatitis,acute necrotizing
hepatic injury
NF - kappa B
splanchnic nerves
