摘要
目的探讨消毒液中的过氧乙酸予以阳离子表面活性剂和增敏动力学光充法的检测价值。方法 2015年1月-2016年12月检测地点为菏泽市立医院,稀硫酸介质下过氧乙酸增强阻抑Fe(Ⅱ)-邻二氮杂菲体系的显色反应,在阻抑体系检测其510 nm波长的吸光度值。结果 0.05～1.50μg/ml范围内检出限为0.02μg/ml,过氧乙酸及吸光度呈现递减式线性关系;在400～600 nm的波长范畴中,非阻抑体系V_(CH3COOOH)=0.00 ml吸收峰为510 nm,尖锐峰形,峰值高,单一峰数,强吸收出现在2.20×10~4 mol吸光系数,在510 nm处吸光谱未发生变化,阻抑体系为最大吸收值,吸光度值在加入过氧乙酸为阶梯式下降;吸光度在阳离子表面活性剂的激活中升高,TPB-STAB混合体系出现明显优势,当TPB、STAB剂量相同时,吸光度突然升高,在2.20×10~4 mol吸光系数吸收波长max。反应时间和吸光度在反应起始时间呈正比例,但其达到最大吸光度值及趋于恒定的时间具有一定的差异;当P_(CH3COOOH)=0μg/ml时,非阻抑体系5～8 min(A值)缓慢上升,9 min后逐渐稳定;当P_(CH3COOOH)=1.0μg/ml时,在1～5 min内阻抑体系A值增高显著,6 min后趋于平稳。结论过氧乙酸对铁(Ⅱ)-邻二氮杂菲分光光度法检测消毒液过氧乙酸效果确切。
【Objective】To study the effect of the sensitization kinetics and the cationic surfactant on detecting the peracetic acid in disinfectant solution.【Methods】From January 2015 to December 2016, samples were tested in Heze Municiple Hospital. The inhibitory effect of peracetic acid in dilute sulfuric acid medium on the chromogenic reaction of Fe（Ⅱ）-two ortho phenanthroline system was very significant, and the absorbance value was detected at the wavelength of 510 nm in the inhibitory system. 【Results】In the range of 0.05～1.50 μg/ml, the linear relationship between the absorbance and peracetic acid was decreased, and the detection limit was 0.02 μg/ml; in the wavelength range of 400-600 nm, the maximum absorption peak appeared at 510 nm in the absorption spectra when VCH3COOOH=0.00 ml（non-inhibitory system）, with a high, sharp, single peak, strong absorption appeared when the molar absorption coefficient was equal to 2.20×104 mol, inhibitory system remained absorption maxima at 510 nm, the shape of absorption spectrum had not changed, after adding peracetic acid the absorbance value stepped down; the absorbance value increased in the activation of the cationic surfactant, the superiority of the TPB-STAB hybrid system was very significant, when the dosage of STAB and TPB was the same, the absorbance value was rising rapidly; the maximum absorption wavelength was at 2.20×104 molar absorptivity. There was a positive correlation between the reaction time and the absorbance value at the initial reaction time, but the difference between the time of reaching the maximum absorbance value and the time of approaching the constant value was significant; when PCH3COOOH=0 μg/ml, in non-inhibitory system, the A value increased slowly for 5-8 minutes, and stabilized after 9 minutes; when PCH3COOOH=1.0 μg/ml, the A value of the inhibitory system increased rapidly in 1-5 minutes, and stabilized after 6 minutes.【Conclusion】The application of Fe（Ⅱ）-two ortho phenanthroline system in the determination of peracetic acid has a good effect.
作者
郑颖华
ZHENG Yinghua(Department of Critical Care Medicine,Heze Municiple Hospital Heze,Shandong 274000,Chin)
出处
《中国医学工程》
2018年第6期23-26,共4页
China Medical Engineering
