摘要
[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.
[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves.[Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction, and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment, Box-Behnken response surface methodology was employed for selecting the optimum extraction process.[Results] The optimum extraction conditions of total flavonoids were as follows： 75% of ethanol concentration, 1∶ 25 of ratio of material to liquid, 31 min of ultrasonic time. Under these conditions, the extraction rate of total flavonoids was 1.115%.[Conclusions] The extraction process obtained by response surface methodology was stable, reasonable, accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.
基金
Supported by the Program of Guilin Municipal Science and Technology Bureau(20130403-4)
Science and Technology Program of Traditional Chinese Medicine from Guangxi Administration of Traditional Chinese Medicine(GZLC14-31)
Undergraduate Innovation and Entrepreneurship Training Plan Project of Autonomous District Level in 2017(201710601082)
