摘要
目的:探讨人乳腺癌细胞MCF-7中mi R-145是否通过调控c-Myc参与肿瘤细胞的生长。方法:化学合成mi R-145-3p和mi R-145-5p并分别转染MCF-7细胞;CCK-8法检测转染后24、48、72 h的细胞增殖情况;RT-PCR检测转染72 h后MCF-7细胞中c-Myc m RNA表达量的变化。结果:与阴性对照组和空白组相比,CCK-8结果显示5p组细胞增殖抑制显著(P<0.05),而3p组抑制效果不明显((P>0.05));RT-PCR结果显示,5p组和3p组细胞中c-Myc m RNA的表达量均下调,且5p组细胞的下调更显著(P<0.05)。结论:乳腺组织中mi R-145负调控c-Myc的表达,可能参与人乳腺癌的发生过程。
Objective：To investigate whether mi R-145 in human breast cancer MCF-7 cells is involved in the growth of MCF-7 cells by regulating c-Myc.Methods：MCF-7 cells were transfected with chemically synthesized mi R-145-3p or mi R-145-5p.Cell Counting Kit-8（CCK-8）assay was used to measure cell proliferation at 24,48,and 72 hours after transfection,and RT-PCR was used to measure the change in the m RNA expression of c-Myc at72 hours after transfection.Results：Compared with the negative control group and blank group,the 5p group had significant inhibition of cell proliferation,while the 3p group did not have marked inhibition,according to CCK-8results.RT-PCR results showed downregulated m RNA expression of c-Myc in both 5p group and 3p group,and the5p group had a significantly greater reduction.Conclusion：mi R-145 significantly downregulates the expression of c-Myc in breast tissue and may thus be involved in the development of human breast cancer.
作者
蔡延森
曾永秋
马明义
税清林
赵矫
CAIYansen;ZENGYongqiu;MAMingyi;SHUIQinglin;ZHAOJiao(Department of Medical Cell Biology and Genetics,SouthwestMedicalUniversity,Luzhou 646000,SichuanProvince,China)
出处
《西南医科大学学报》
2018年第3期198-200,共3页
Journal of Southwest Medical University
基金
泸州市科技局项目(2013-S-47(20/20))
泸州医学院院级项目[2010]108号
