摘要
目的:探讨加味柴芍六君方联合壮医药线点灸干预胃癌前病变模型大鼠的作用机制。方法:将120只健康清洁级雄性Wistar大鼠随机分为6组,即空白对照组、模型组、胃复春对照组、中药低剂量组、中药中剂量组及中药高剂量组,均20只。空白对照组正常进食水,其他5组采用综合法造胃癌前病变模型。造模成功后,各组给予对应的药物干预(中药组同时配合壮医药线点灸外治)后断头处死取胃。采用免疫组织化学S-P法检测脾酪氨酸激酶(spleen tyrosine kinase,Syk)、突变型p53蛋白及生存素(Survivin)的表达水平,观察胃黏膜组织病理,分析其干预胃癌前病变的作用机制。结果:药物干预期间,中药中剂量组、中药高剂量组大鼠毛色发黄,大便溏结不调,耳朵黏膜色泽差,进食量减少,精神萎靡,反应迟钝等症状基本消失,干预6周后恢复正常;胃复春对照组及中药低剂量组上述症状有所改善,但改善效果没有中药中剂量组、中药高剂量组明显;和模型组相比,胃复春对照组、中药低剂量组、中药中剂量组、中药高剂量组大鼠的体质量均明显升高,差异具有统计学意义(P<0.05);中药中剂量组、中药高剂量组大鼠体质量明显高于胃复春对照组及中药低剂量组,差异具有统计学意义(P<0.05);和模型组相比,中药高剂量组大鼠腺体萎缩、肠上皮化生发生情况均明显减少,差异具有统计学意义(P<0.05);和模型组相比,胃复春对照组、中药中剂量组、中药高剂量组大鼠Syk蛋白阳性表达情况明显增加,阴性表达情况明显减少,差异具有统计学意义(P<0.05);和胃复春对照组相比,中药高剂量组大鼠Syk蛋白阳性表达情况明显增加,阴性表达情况明显减少,差异具有统计学意义(P<0.05);和模型组相比,胃复春对照组、中药中剂量组、中药高剂量组大鼠Survivin蛋白阳性表达情况明显减少,阴性表达情况明显增加,差异具有统计学意义(P<0.05);和胃复春对照组相比,中药高剂量组大鼠Survivin蛋白阳性表达情况明显减少,阴性表达情况明显增加,差异具有统计学意义(P<0.05);和模型组相比,胃复春对照组、中药高剂量组大鼠突变型p53蛋白阳性表达情况明显减少,阴性表达情况明显增加,差异具有统计学意义(P<0.05)。结论:对胃癌前病变模型大鼠应用加味柴芍六君方联合壮医药线点灸治疗,可显著提高治疗效果,而增强Syk蛋白的表达和减弱Survivin蛋白、突变型p53蛋白的表达,可能是其作用机制。
Objective：To investigate the effect of moxibustion of Chaishao Liujun prescription combined with Zhuang medicine line moxibustion on precancerous lesions of gastric cancer rats. Methods：120 healthy male Wistar rats were randomly divided into 6 groups：blank control group,model control group,Weifuchun control group,traditional Chinese medicine low dose group,traditional Chinese medicine middle dose group and traditional Chinese medicine high dose group,with 20 rats in each group. The blank control group was fed normal water,and the other 5 groups were treated after the model of comprehensive precancerous lesions of gastric cancer（ PLGC） being established： each group was given a corresponding drug intervention（ Chinese medicine group with Zhuang medicine line point moxibustion external treatment） after the decapitation of the stomach. Immunohistochemical S-P method was used to detect the expression of spleen tyrosine kinase（ Syk）,mutant p53 protein and survivin. Observe the histopathology of gastric mucosa and analyze its mechanism of intervention on gastric precancerous lesions. Results：During the drug intervention period,the rats in the middle-dose group and high-dose group of traditional Chinese medicine showed yellow hair color,loose bowel movements,poor color of the mucous membranes of the ears,decreased food intake,apathetic,and unresponsive symptoms. The symptoms disappeared after 6 weeks of intervention. Normal;The symptoms of Weifuchun control group and low dose of traditional Chinese medicine group improved,but the improvement effect was not obvious in the medium-dose group and high-dose group;the model group had yellow hair,loose stool,poor color of the mucous membranes of the ears,eating Decreased quantity,apathetic and unresponsive;Compared with the model group,the body weight of the rats in the Weifuchun control group,the low-dose group,the middle-dose group,and the high-dose group were significantly higher,and the difference was statistically significant（ P〈0. 05）;The body weight of rats in the medium-dose group and high-dose group was significantly higher than that in the control group and the low-dose group,the difference was statistically significant（ P〈0. 05）;Compared with the model group,the high dose of the traditional Chinese medicine The number of cases of glandular atrophy and intestinal metaplasia decreased significantly（ P〈0. 05）. Compared with the model group,Weifuchun control group,Chinese medicine medium dose group,and high dose The number of positive Syk protein expression in the rats of the quantitative group was significantly increased,and the number of negative expression was significantly reduced（ P〈0. 05）. Compared with the Weifuchun control group,the Syk protein expression was positive in the rats of the high dose group. The number of cases increased significantly,and the number of negative expression cases decreased significantly（ P〈0. 05）. Compared with the model group,the positive expression of Survivin protein in the rats in the Weifuchun control group,the middle-medium dose group,and the high-dose group The number of negative cases was significantly decreased,and the number of negative cases was significantly increased（ P〈0. 05）. Compared with the Weifuchun control group,the number of cases of positive expression of Survivin protein in the high dose group of Chinese herbs was significantly reduced,and the number of negative cases was significantly decreased（ P〈0. 05）;Compared with the model group,the positive expression of p53 protein in the rats in the Weifuchun control group and the high-dose Chinese herbs group was significantly reduced,and the negative expression was significantly increased,and the difference was statistically significant（ P〈0. 05）. Conclusion：The treatment of gastric precancerous lesion model rats with modified Chaishao Liujun Prescription combined with Zhuang Medicine line moxibustion can significantly improve the therapeutic effect,the possible mechanism of which is enhancing the expression of Syk protein and weakening the expression of Survivin protein and p53 protein during the process.
作者
蒋敏玲
范剑薇
江伟
JIANG Minling;FAN Jianwei;JIANG Wei(The Affiliated Hospital to Guilin Medical College,Guilin Guangxi China 541515)
出处
《中医学报》
CAS
2018年第7期1159-1165,共7页
Acta Chinese Medicine
基金
广西壮族自治区自然科学基金面上项目(2012GXNSFAA053078)
关键词
胃癌前病变
加味柴芍六君方
壮医药线点灸
大鼠
肿瘤
Gastric precancerous lesions
modified Chaishao Liujun Prescription
Zhuang medicine line moxibustion
rat
tumor
