摘要
目的为提高速测结果的准确性,开展蔬菜定性检测假阴性风险研究。方法采用酶抑制率法对351169批次蔬菜样品进行定性检测,针对检出的406批次40%≤抑制率<50%蔬菜样品采用色谱法对有机磷和氨基甲酸酯两类农药进行定量确证。结果 40%≤抑制率<50%蔬菜样品总体假阴性率高达25.4%,其中鳞茎类、叶菜类和茄果类假阴性数量和假阴性率明显较高,菠菜、马兰、香芹菜、石刁柏4种蔬菜假阴性率达到100%,超标农药中以禁用农药克百威和毒死蜱为主,且检出多种农药并存。结论应用酶抑制率法快速检测蔬菜农药残留存在一定的假阴性风险,建议以酶抑制率≥40%作为初筛判定标准,进而采用气相色谱法和液相色谱法进行定量确证,可以有效降低假阴性率。
Objective To investigate the false-negative risk of pesticide residues in vegetable, so as to improve the rapid detection accuracy. Methods The enzyme inhibition rate method was used to qualitatively detect 351 169 batches of vegetable samples, and 406 batches of vegetable samples with the inhibition rate within 40%-50% were conducted for the quantification detection of organophosphate and carbamate residues by chromatography. Results The total false-negative rate of those redetected vegetable samples was 25.4% , of whieh the false-negative count and the false-negative rate in bulbous, leafy and solanceous vegetables were higher. The false-negative rate in spinach, Kalimeris indica, parsley and Asparagus offieinalis were up to 100%. Among the over-standard pesticides, earbofuran and chlorpyrifos, the banned pesticides, were the dominant, and multiple pesticides were detected. Conclusion There is a certain risk of false-negative for the enzyme inhibition method to detect the pesticides residues in vegetables, so it is recommended to use the enzyme inhibition rate ≥40% as the screening criteria, and gas chromatography and liquid chromatography are used for the quantitative confirmation, which can effectively reduce the false negative rate.
作者
张瑜
魏维杰
李岗
魏云潇
邵康群
ZHANG Yu;WEI Weijie;LI Gang;WEI Yunxiao;SHAO Kangqun(Yuhang Food and Drug Monitoring and Testing Center,Hangzhou,Zhejiang 3l1199,China)
出处
《中国卫生检验杂志》
CAS
2018年第14期1760-1763,共4页
Chinese Journal of Health Laboratory Technology
关键词
蔬菜
农药
酶抑制率
假阴性
色谱法
Vegetable
Pesticide
Enzyme inhibition rate
False negative
Chromatography
