摘要
目的:观察下调肺癌细胞中STMN1基因的表达对癌细胞增殖、侵袭及PI3K/AKT信号通路的影响。方法:以人正常肺细胞MRC-5作为对照,通过Western bloting检测人肺癌A549、SPC-A1和H322细胞中STMN1的蛋白表达;STMN1的siRNA转染A549细胞为实验组,另设空白组和阴性组,转染48 h后,Western bloting检测转染效果;CCK8法及Transwell小室分别检测细胞的增殖及侵袭能力;Western bloting检测增殖细胞核抗原(PCNA)、基质金属蛋白酶2(MMP-2)、磷脂酰肌醇3-激酶(PI3K)、磷酸化的丝氨酸苏氨酸激酶(p-AKT)的蛋白表达。结果:STMN1在肺癌A549、SPC-A1和H322细胞中的蛋白表达均显著高于在MRC-5细胞中的表达(P<0.05),选择A549细胞为研究对象。转染STMN1 siRNA的A549细胞STMN1的蛋白表达显著低于空白组(P<0.05)。与空白组比较,实验组细胞增殖及侵袭能力均显著降低,PCNA、MMP-2、PI3K、p-AKT蛋白表达均显著降低(P<0.05)。结论:siRNA沉默STMN1的表达通过下调PI3K/AKT信号通路降低肺癌细胞增殖及侵袭能力。
Objective： To observe the effect of down-regulation of STMN1 expression on proliferation,invasion and PI3 K/AKT signaling pathway in lung cancer cells. Methods： Normal human lung cell MRC-5 as control,STMN1 protein expression in human lung cancer A549,SPC-A1 and H322 cells were detected by Western bloting. STMN1 siRNA transfected with A549 cells as experimental group,and the blank and negative groups were set,cells were transfected for 48 h,the transfection effects were detected by Western bloting. The proliferation and invasion ability of the cells were detected by CCK8 and Transwell respectively. The protein expression of PCNA,MMP-2,PI3K and p-AKT were detected by Western bloting. Results： The expression of STMN1 proteins in lung cancer A549,SPC-A1 and H322 cells were significantly higher than the expression in MRC-5 cells（ P〈0. 05）,A549 cell was selected as the research object. The expression of STMN1 protein in A549 cells transfected with STMN1 siRNA was significantly lower than that in the blank group（ P〈0. 05）. Compared with the blank group,the proliferation and invasion of cells were significantly decreased,the expression of PCNA,PI3K,MMP-2 and p-AKT protein were significantly decreased（ P〈0. 05）. Conclusion： Silencing of STMN1 expression by siRNA reduced the proliferation and invasiveness of lung cancer cells by down regulation of PI3K/AKT signaling pathway.
作者
崔青荣
杨秦梅
李彬
李宁
Cui Qingrong;Yang Qinmei;Li Bin;Li Ning(Department of Respiration Ⅲ;Department of Respiration Ⅱ;Department of Respiratory Medicine,The First Affiliated Hospital of Henan University of Chinese Medicine,Henan Zhengzhou 450000,China)
出处
《现代肿瘤医学》
CAS
2018年第14期2143-2146,共4页
Journal of Modern Oncology
基金
国家自然科学基金青年项目(编号:81501991)
