摘要
目的研究柏子仁甙(semen platycladi saponins,SPS)对阿尔茨海默病(Alzheimer’s disease,AD)模型鼠海马氧化应激反应的影响,进一步探讨SPS对AD模型鼠的神经保护机制。方法将大鼠分为正常对照组、模型组和SPS干预组,模型组和SPS干预组应用β淀粉样蛋白1-42(β-amyloid 1-42,Aβ1-42)于大鼠双侧海马注射制作AD动物模型。模型成功后,正常对照组和模型组给予生理盐水5 mL+羧甲基纤维素钠(500 mg/kg)灌胃,持续30 d;SPS干预组每天给予生理盐水5 mL+SPS(300 mg/kg)灌胃,持续30 d,然后应用Morris水迷宫实验测定模型鼠的学习与记忆功能,应用生物化学方法检测SPS对模型鼠海马脑组织超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽(glutathione,GSH)、丙二醛(malondialdehyde,MDA)的影响。应用Western blotting印迹技术观察AD模型鼠海马Bcl-2、survivin、Fas、Bax、caspase-3蛋白及mRNA的表达。结果模型组搜索时间与另外两组相比延长(P<0.01);搜索距离百分比降低(P<0.01);SPS干预组较正常对照组搜索时间明显延长(P<0.01)、搜索距离百分比明显降低(P<0.01)。SPS干预组MDA较模型组明显减低(P<0.01),但较正常对照组升高(P<0.01);SPS干预组SOD、GSH较模型组明显升高(P<0.01),但较正常对照组降低(P<0.01)。SPS干预组较模型组Bcl-2和survivin明显升高(P<0.01),但较正常对照组降低(P<0.05);SPS干预组较模型组Fas、Bax、caspase-3明显降低(P<0.01),但较正常对照组升高(P<0.05)。结论 SPS可以通过抑制AD模型鼠海马氧化应激反应,提高抗氧化系统活性,从而改善AD模型鼠的认知功能,对AD模型鼠具有神经保护作用。
Objective To study the influence of semen platycladi saponins on oxidative stress response of hippocampus of rat model of Alzheimer’ s disease （AD）, and to explore the neuroprotective mechanism of semen platycladisaponins （SPS） on AD model rats. Methods Sixty SPF 24-week old female Wistar rats were divided into three groups：the normal control group, AD model group, and SPS intervention group. The rats of AD model group and SPS group wereinjected with Aβ1-42 in bilateral hippocampi to produce AD animal models. After the successful establishment of AD model,the AD model group and the normal control group received 5 mL saline sodium with carboxymethyl cellulose （500 mg / kg）orally, daily for 30 days. The SPS group received orally 5 mL normal saline with 300 mg / kg SPS daily for 30 days. Thelearning and memory function of the rats were assessed by Morris water maze test, and the levels of MAD, SOD and GSH inthe hippocampal tissues of model rats were detected with biochemistry. The expressions of Bcl-2, survivin, Fas, Bax,caspase-3 proteins and mRNA in the hippocampi of AD model rats were detected by western blotting. ResultsCompared with the control group and SPS group, the rats in AD model group displayed a longer search time and shorterpercentage of search distance （P 〈 0.01）. There was a longer search time and lower percentage of search distance of theSPS groups than the control group （P 〈 0.01）. The positioning experiment showed that rats in the control group learned tofind the platform within 2- 1 days, indicating that the latency was rapidly decreased. MDA in the SPS intervention groupwas significantly decreased than in the AD model group （P 〈 0.01）, but increased than the normal control group （P 〈0.01）. The expressions of SOD and GSH were significantly increased in the SPS group than the model group （P 〈 0.01）.The expressions of Bcl-2 and survivin were significantly increased in the SPS group than the model group （P 〈 0.01）, butlower than the normal control group （P 〈 0.05）. The expressions of Fas, Bax and caspase-3 were decreased in the SPSintervention group （ P 〈 0.01 ）, but increased than the normal control group （ P 〈 0.05 ）. Conclusions Semenplatycladi saponins can protect the neurons and improve the cognition function of AD model rats by inhibiting oxidativestress response and enhancing the antioxidant mechanism in the hypocampus.
作者
索金红
牟海军
刘晓娟
姚福祥
杨文广
SUO Jinhong;MOU Haijun;LIU Xiaojuan;YAO Fuxiang;YANG Wenguang(Tongliao Vocational College,Inner Mongolia,Tongliao 028000,China;Department of RespiratoryDiseases,Third Affiliated Hospital,Qiqihar Medical College,Qiqihaer 161000;Department of Pharmacy,Tongliao Hospital,Inner Mongolia,Tongliao 028000)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第6期84-88,95,共6页
Chinese Journal of Comparative Medicine
关键词
阿尔茨海默病
柏子仁甙
丙二醛
超氧化物歧化酶
谷胱甘肽
Alzheimer＇ s disease
semen platycladi saponins
malondialdehyde
superoxide dismutase
glutathione
