摘要
微生物通过参与生物地球化学循环物质循环、污染物的降解转化等过程对维持河流生态环境的稳定起着主导作用。利用放射性同位素示踪复杂环境中微生物生物量的周转速率,能更直接地揭示原位环境中微生物的活性,其可作为水体微生物营养物质代谢和有机污染物降解速率的潜在指标。作者重点探讨了以3H-亮氨酸为基础的同位素示踪法测定水体环境微生物生物量碳周转速率的技术原理和应用。以嘉陵江(南充市段)为研究对象,结果表明:(1)该河段不同采样点处表层水样中微生物生物量碳周转速率有显著差异(577.89~2091.70 n M C L-1d-1),城市支流西河显著高于嘉陵江干流,可能由于此处较高的生活污水污染的影响所致。(2)相关性分析表明嘉陵江(南充市段)表层水样中的微生物生物量碳周转速率与水样中铵态氮浓度和微生物丰度成显著正相关(P<0.05),与水样中硝态氮浓度成显著负相关(P<0.05)。本文为同位素介导的水体环境微生物代谢活性测定提供了快速高效的方法参考。
Microorganisms inhabiting river ecosystems are one of the crucial players in the maintenance of ecosystem health and balance by its biogeochemical cycling of organic matter and nutrients,biodegradation and biotransformation of pollutants. The radioactive isotope tracer method of microbial biomass turnover rate can directly reveal the in situ activity of microorganism and work as a potential indicator of nutrients metabolisms and organic pollutants biodegradation rate. By taking the Nanchong section of Jialing River as the object of study,this paper mainly discusses about the principle and application of ^3H-Lecuine based isotope tracing method in determining the microbial biomass turnover rate in river ecosystems. The result reveals distinct differences in surface water microbial biomass turnover rate from 577. 89 to 2091. 70 n M C L^-1 d^-1. The highest microbial biomass turnover rate in urban branch（Xi River） is possibly caused by the high input of domestic sewage. Correlation analysis indicates that NH4^＋-N content and microbial abundance of the surface water are positively correlated to microbial biomass turnover rate（P〈0. 05） while NO3^--N content is negatively related to microbial biomass turnover rate（P〈0. 05）. This study provides a fast and efficient method to measure the environmental microbial activity.
作者
路璐
LU Lu(College of Environmental Science and Engineering,China West Normal University,Nanehong Siehuan 637009,China)
出处
《西华师范大学学报（自然科学版）》
2018年第2期137-142,共6页
Journal of China West Normal University(Natural Sciences)
基金
国家自然科学基金项目(41606142)
西华师范大学英才科研基金项目(463140)
西华师范大学博士启动基金项目(412554)
