摘要
目的观察不同的脱蜡剂对实时荧光定量PCR检测表皮生长因子受体(EGFR)基因结果判读的影响。方法从2014年11月起,连续6个月收集该院病理科石蜡标本切片68份,均分成A组(二甲苯脱蜡)和B组(松节油脱蜡)进行石蜡块EGFR基因突变检测。结果同一份石蜡切片检测EGFR的6个靶点两组结果均相同。两组同一份标本的内质控(内参)扩增曲线CT值之差均小于0.99;同一份标本,两组检测相同靶点时有效的扩增曲线CT值之差均小于0.99,且结合内质控(内参)CT值分析最终判定两组靶点检测的阳性、阴性结果完全相符。结论采用松节油与二甲苯脱蜡后检测EGFR基因突变的实时荧光定量PCR结果一致,对检测的靶点无影响,松节油可用作分子实验室的脱蜡剂。
Objective To observe the effects of different dewaxing agents on the detection of epidermal growth factor receptor（EGFR）gene mutation in paraffin-embedded tissues specimens by real-time quantitative PCR.Methods From November 2014,68 paraffin-embedded specimens were were consecutively collected from the department of pathology in this hospital for 6 months.All specimens were each treated with different dewaxing agents：the group A was treated with xylene dewaxing,the group B was treated with turpentine dewaxing.Then DNA was extracted by DNA extraction kit and detected by real-time quantitative PCR.ResultsThe results of 6 targets of EGFR in each paraffin-embedded section were consistent for the two groups.For each paraffin-embedded section,the difference of CT value of interval reference amplification curve between the group A and the group B was less than 0.99.For the same targets of each paraffin-embedded section,the difference of CT values of effective amplification curve between the group A and the group B were less than 0.99.Considering the CT value of internal control（internal reference）,the positive and negative results of targets in group A were consistent with those in group B.Conclusion The results of epidermal growth factor receptor（EGFR）gene mutation in turpentine dewaxing paraffin-embedded tissues specimens are consistent with those in xylene dewaxing paraffin-embedded tissues specimens by real-time quantitative PCR,without influences on detection results of each target,which indicates turpentine could serve as an alternative dewaxing agent for molecular laboratory.
作者
徐帆
XU Fan(Department of Pathology,Chongqing Three Gorges Central Hospital,Chongqing 400400,Chin)
出处
《重庆医学》
CAS
2018年第18期2467-2469,共3页
Chongqing medicine
关键词
石蜡
表皮生长因子受体基因
实时荧光定量PCR
检测靶点
脱蜡剂
实验室环境
paraffin；epidermal growth factor receptor genes；real-time quantitative PCR；detection target；dewaxing agent；laboratory environment
