摘要
本实验建立了固相萃取-超高效液相色谱(SPE-UPLC)方法测定茶叶中柠檬黄、新红、苋菜红、胭脂红、日落黄和亮蓝等6种着色剂,该方法具有快速、高效的特点。在检测波长254nm,甲醇-0.02mol/L乙酸铵的梯度洗脱条件下,6种着色剂的线性良好(r2>0.99),加标回收率为78.4%~118%,RSD均小于3%,检出限为0.1~1.0mg/kg,定量限为0.3~3.0mg/kg。用建立的方法对市售茶叶进行了检测。
In this experiment,a ultra performance liquid chromatography with solid phase extraction（SPE-UPLC）method was built to determine 6 synthetic colorants,including tartrazine,new red,amaranth,ponceau 4 R,sunset yellow and brilliant blue in tea,which was rapid,accurate and efficient.Methanol-ammonium acetate（0.02 mol/L）was chosen as mobile phase,gradient elution was used in the determination and the detection wavelength was 254 nm.6 synthetic colorants showed good linearities（r20.99）in this condition,the average recoveries were between 78.4%and 118% both at high and low spiked levels,relative standard deviations（RSDs）3%.The limits of detection（LOD）were in the range of 0.1~1.0 mg/kg,and limits of quantitation（LOQ）were in range of 0.3~3.0 mg/kg.The developed method has been used in the detection of 6 synthetic colorants in market tea.
作者
石伟杰
范素芳
曹梅荣
贾文轩
张岩
SHI Wei-jie;FAN Su-fang;CAO Mei-rong;JIA Wen-xuan;ZHANG Yan(Hebei Food Inspection and Research Institute,Key Laboratory of Food Safety of HebeiShijiazhuang Hebei 050227,China)
出处
《河北省科学院学报》
CAS
2018年第2期80-86,共7页
Journal of The Hebei Academy of Sciences
基金
河北省食品药品监督管理局食品安全科技计划项目(ZD2015001)
关键词
固相萃取
超高效液相色谱
茶叶
着色剂
Solid phase extraction
Ultra performance liquid chromatography
Tea
Synthetic colorants
