摘要
背景:有研究显示脱发区域中的毛囊干细胞并未消失,只是处于异常的休止状态而无法产生毛发。目的:通过观察重组人生长素对体外毛囊培养中的效果,以证实重组人生长素对毛发再生的有效性,并拟从蛋白表达角度探讨可能的作用机制。方法:将分离完整的毛囊随机分成实验组与对照组,实验组分别置入重组人生长素浓度分别为0.01,0.1,1,10,20与40 IU/L无血清毛囊生长培养液中,对照组则置入不含生长素的培养液中。每孔放入6根完整毛囊,置37℃、体积分数5%CO2培养箱中培养,在倒置显微镜下用目镜测微尺每天测量每根毛囊的长度并记录;并检测应用生长素后的生长因子表达。结果与结论:(1)与对照组相比,实验各组不同时间段毛囊生长速度均有不同程度的增快,有一定的浓度依赖性,10 IU/L时达到较好效果浓度,随后减弱。1 IU/L组、10 IU/L组生长长度显著长于对照组(P<0.05);(2)生长素毛囊生长曲线显示,各组均呈不同角度向上,0.1、40 IU/L组效果相近,而且6 d前生长斜度最大,6 d后生长斜度减小;(3)蛋白成分分析显示,应用生长因子后成纤维细胞生长因子、转化生长因子、肿瘤坏死因子β与白细胞介素21表达下降;表皮生长因子、胰岛素样生长因子2、神经生长因子、肝细胞生长因子、干细胞因子等蛋白因子均表达增强;(4)结果说明,重组人生长素对毛囊的生长有较好的促进作用,可能是通过促进激活多种促毛囊生长因子起作用。
BACKGROUND： Alopecia has been shown to be caused by the abnormally inactive state of hair follicle stem cells but not their death. OBJECTIVE： To confirm the effectiveness of recombinant human growth hormone（rh GH） in promotion of hair regeneration by observing rh GH acting on the hair follicle cultured in vitro, and to explore the possible mechanism at protein level.METHODS： The individual hair follicle was separated and was randomized into two groups, and then cultured in the serum-free medium containing 0.01, 0.1, 1, 10, 20 and 40 IU/L rh GH, respectively. The cells were cultured in the normal medium served as the control growp. Six individual hair follicles were added into each pore, and incubated in an incubator at 37 ℃ and 5% CO2. Its length was measured using ocular micrometer under inverted contrast microscope daily. Additionally, the expression levels of growth factors were tested after rh GH application. RESULTS AND CONCLUSION： Compared with the control group, the growth speed of hair follicle was increased at different degrees after different concentrations of rh GH was applied. The optimal concentration of rh GH was 10 IU/L, and then decreased. The length of hair follicle in the 1, and 10 IU/L groups was significantly longer than that in the control group（P〈0.05）. The growth curve of hair follicle showed a rising tendency in each group, and 0.1 and 40 IU/L rh GH revealed the similar effect. The largest growth gradient appeared before day 6, followed by a slight increase. rh GH downregulated the expression levels of fibroblast growth factor, transforming growth factor, tumor necrosis factor β and interleukin-21, and upregulated the levels of epidermal growth factor, insulin-like growth factor 2, nerve growth factor, hepatocyte growth factor, and stem cell factor. In summary, rh GH exerts a positive effect on the growth of hair follicle possibly by activating a variety of related growth factors.
作者
张友来
辛国华
曾元临
江澜
王晓
邹立津
Zhang You-lai;Xin Guo-hua;Zeng Yuan-lin;Jiang Lan;Wang Xiao;Zou Li-jin(Department of Burn,the First Affiliated Hospital of Nanchang University,Nanchang 330006,Jiangxi Province,Chin)
出处
《中国组织工程研究》
CAS
北大核心
2018年第20期3167-3171,共5页
Chinese Journal of Tissue Engineering Research
基金
江西省卫计委科技支撑计划(12010637)~~
关键词
重组人生长激素
毛囊
生长因子
脱发
组织构建
Human Growth Hormone
Hair Follicle
Cytokines
Alopecia
Tissue Engineering
