摘要
运用响应面法优化了鼠李糖脂水解制备鼠李糖的反应条件。在单因素实验的基础上,根据Box-Behnken中心组合试验设计原理采用3因素3水平的响应面分析法,研究了水解过程中硫酸用量、反应温度以及反应时间对鼠李糖脂水解收率的影响,拟合了水解收率与3因素之间的回归方程,对鼠李糖脂的水解反应条件进行了优化。研究结果表明,硫酸用量以及反应温度对鼠李糖脂水解收率的影响较显著,硫酸用量与反应时间以及反应温度与反应时间之间的交互作用影响显著,各因素的二次方影响极显著。由模型得出的最优条件为:硫酸用量2.3%(质量分数)、反应温度137℃、反应时间2.8 h。在此条件下,鼠李糖脂水解制备鼠李糖的收率达81.6%,与预测值82.35%较为吻合。最后,将模型进一步应用于3 m3工业规模的水解生产中,发现预测误差在-8.12%~9.30%,说明该模型对工业生产具有较好的参考价值。
The response surface methodology（RSM） was used to optimize the hydrolysis reaction conditions of rhamnolipids for preparation of rhamnose. On the basis of single factor experiments, three-factor response surface analysis method was used to study the hydrolysis yield of rhamnolipid in the hydrolysis process using the amount of sulfuric acid, reaction temperature, and reaction time as three-levels according to the Box-Behnken central combination design principle. The regression equation between the hydrolysis yield and three factors was fitted to optimize the hydrolysis reaction conditions of rhamnolipids. The results showed that the amount of sulfuric acid and the reaction temperature had significant effect on the hydrolysis of rhamnolipid. The interaction between the amount of sulfuric acid and hydrolysis time, and the interaction between hydrolysis temperature and time both have significant effects. The quadratic effects of various factors were extremely significant. The optimal hydrolysis conditions derived from the model were： 2.3%（mass fraction） of sulfuric acid, hydrolysis temperature of 137 ？C and the hydrolysis time of 2.8 h. Under these conditions, the actual yield of rhamnose was 81.6%, which was in agreement with the predicted value of 82.35%. Finally, the model was futher applied to 3 m3 industrial-scale hydrolysis production and the prediction error was found to be in between-8.12% and 9.30%, indicating that the model had a good reference value for industrial production.
作者
周超俊
沈冲
程层
孟琴
Zhou Chaojun;Shen Chong;Cheng Ceng;Meng Qin(College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China)
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
2018年第1期65-72,共8页
Chemical Reaction Engineering and Technology
基金
国家自然科学基金(21776242)
国家重点研发计划(2016YFD0400801)
关键词
鼠李糖
鼠李糖脂
水解
响应面法
rhamnose
rhamnolipids
hydrolysis
response surface methodology
