摘要
胶孢炭疽菌是一种病原菌,它可以引起炭疽病进而侵扰多种农作物,造成相当严重的经济损失。因此为了进一步地了解这个病菌的分子致病的机理,本研究从胶孢炭疽菌当中克隆出一个新的寡肽转运蛋白基因,并将它命名为CgOPT2,通过对生物信息学的分析我们发现这个基因编码一个990个氨基酸的蛋白,含有15个跨膜区,共同地组成了OPT结构域。为了得到此基因的敲除突变体,我们就可以采用同源重组的办法,通过把突变体还有野生型的各项进行表格的对比处理,一般来说,突变体具体表现为生长缓慢,菌丝比较稀疏而且它的疏水性增强,孢的产量低,对H_2O_2和SDS更为敏感,致病力更为减弱。通过上述的结果可以发现,CgOPT2主要参与对胶孢炭疽菌的营养发育调控,分生孢子的产生、致病性还有氧化应激反应等。
Colletotrichum gloeosporioides is an important pathogen,which can cause anthracnose and infect numerous crops,causing serious economic losses.In order to further understand the molecular pathogenic mechanism of the pathogen,a new oligopeptide transporter gene CgOPT2 was cloned from C.gloeosporioides in this study,which encodes a 990-amino acids protein,containing an OPT domain including 15 transmembrance domains.The gene knockout mutant of CgOPT2 was obtained by homologous recombination.By comparing several aspects of the mutant and wild type,the results showed that mutant was characterized by slow growth,sparse aerial hyphae with increased hydrophobicity,decreased conidium production,more sensitive to H_2O_2 and SDS,significantly decreased virulence.These results demonstrated that CgOPT2 might be involved in regulation of vegetative growth,conidium production,pathogenicity,and oxidative stress response.
作者
张楠
胡坚
柳志强
李晓宇
Zhang Nan;Hu Jian;Liu Zhiqiang;Li Xiaoyu(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,57022)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第8期3387-3393,共7页
Genomics and Applied Biology
基金
国家自然科学基金(31560045)
海南省重点研发计划(ZDYF2016155)共同资助
关键词
胶孢炭疽菌
寡肽转运蛋白
分生孢子化
致病性
Colletotrichum gloeosporioides
Oligopeptide transporter
Conidium
Pathogenicity
