摘要
目的探讨雌激素调节阴道上皮细胞对2种阴道常驻菌的天然免疫状态的影响,揭示萎缩性阴道炎潜在的发病机制。方法以阴道上皮细胞株VK2/E6E7为模型,在不同雌激素环境下,分别给予不同浓度阴道奇异菌和弯曲乳酸杆菌进行体外刺激,酶联免疫吸附法(ELISA)测定培养上清液中炎性细胞因子[白介素(IL)-1a、IL-1b、IL-6、肿瘤坏死因子(TNF)a]和趋化因子IL-8的分泌水平。结果阴道奇异菌诱发阴道上皮出现显著天然免疫反应,且IL-6和IL-8分泌呈现出一定程度的剂量依赖特性,菌液浓度为4×10~6 CFU/m L,刺激12 h时IL-6为(176.562±11.991)ng/L,IL-8为(1 049.883±121.348)ng/L;而阴道弯曲乳酸杆菌并未诱发阴道上皮细胞株产生明显的天然免疫反应。在雌激素环境下,阴道上皮细胞对阴道奇异菌刺激所产生免疫反应的强度显著变弱:但IL-8的分泌时间显著延迟到刺激后4 h,且IL-6和IL-8的最大上升幅度显著下降(IL-6,P=0.001 9;IL-8,P=0.008 7)。结论奇异菌能够诱导阴道上皮以剂量依赖的方式分泌炎性细胞因子;同时,雌激素能调节IL-6和IL-8的分泌,从而调节后续的保护机制,拮抗病原微生物的入侵和过度生长,维持阴道的健康。
Objective To explore the effects of estrogen regulating vaginal epithelial cells on the natural immune status of two kinds of vaginal resident bacteria, and to reveal the pathogenesis of atrophic vaginitis potential. Methods Vaginal epithelial cell lines VK2/E6 E7 were treated as model. Under the different environmental estrogen, different concentrations of Atopobium and Lactobacillus curvatus were given to in vitro stimulate vaginal epithelial cell lines. Enzyme-linked immunosorbent assay（ELISA） method was used to determine the secretion of inflammatory cytokine and chemokine. Results Vaginal epithelium induced a significant natural immune response, and the secretion of interleukin-6（IL-6） and IL-8 presented a certain amount of dosedependent characteristics. However, Lactobacillus crispatus did not induce innate immune response in vaginal epithelial cell lines. In the estrogen environment, the strength of the immune response of vaginal epithelial cells in the stimulation of Atopobium vaginae was significantly changed. IL-8 secreted time delayed, the maximum increase of IL-6 and IL-8 decreased significantly（IL-6, P=0.001 9; IL-8, P=0.008 7）. Conclusion Atopobium vaginae can induce the secretion of inflammatory cytokines in the vaginal epithelium by dose-dependent manner. At the same time, estrogen regulates the secretion of IL-6 and IL-8 synthesis, at relatively low and manageable level, adjust the subsequent protection mechanism of disease-resistant original microbial growth, invasion and maintaining the health of the vagina.
作者
许啸声
沈健
刘延
唐海婷
Xu Xiaosheng;Shen Jian;Liu Yan;Tang Haiting(Department of Obstectrics and Gynecological,Ruijin Hospital Affiliated to Medical School of Shanghai Jiao Tong University,Shanghai 200025,Chin)
出处
《中华生殖与避孕杂志》
CAS
CSCD
北大核心
2018年第6期497-503,共7页
Chinese Journal of Reproduction and Contraception
关键词
萎缩性阴道炎
阴道上皮
天然免疫
雌激素
Atrophic vaginitis
Vaginal epithelium
Natural immunity
Estrogen
