摘要
利用12个微卫星标记对鲤(Cyprinus carpio)的4个野生群体[清水江鲤、太湖鲤、黄河鲤(C.carpio haematopterus)和黑龙江鲤(C.carpio amurensis)]和2个选育群体[福瑞鲤(C.carpio var.FFRC)和松浦镜鲤(C.carpio var.specularis'Song-pu')]共208尾个体进行遗传分析。结果显示,12个位点共检测到341个等位基因,平均等位基因数为28.67,其中1个位点(HLJ1127)检测到正向选择压力;选育群体的遗传多样性参数普遍低于野生群体,其中松浦镜鲤群体的各项参数均值最低(Na=6.82,Ho=0.54,PIC=0.50),清水江鲤群体的各项参数均值最高(Na=21.25,Ho=0.80,PIC=0.91);分子方差分析显示,整体遗传变异主要来自群体内,但群体间呈极显著遗传分化(P<0.01);基于群体Nei's遗传距离的UPGMA聚类树和PCo A分析表明,鲤4个野生群体间遗传距离较近,而与2个人工选育群体间遗传距离较远;基于个体遗传结构及PCo A分析显示部分野生个体遗传结构比较混杂,而选育个体的遗传结构则相对单一。研究表明,中国鲤野生资源具有较高的遗传多态性,而人工选育群体维持着较纯的遗传种质。
We genotyped a total of 208 individuals of common carp （Cyprinus carpio） from four wild populations [Qingshuijiang （QSJ）, Taihu （TH）, C.carpio haematopterus （Huangheli, HHL） and C.carpio amurensis （Heilongjiang, HLJ）] and two selected strains [C.carpio var. FFRC （FRL） and C.carpio var. specularis ＇Song-pu＇ （SPJ）] by using 12 microsatellite markers. Altogether 341 alleles were detected in 12 loci, with an average of 28.67 alleles per locus, and one locus （HLJ1127） was under strong selection pressure. Two selection strains showed lower genetic diversity parameters values than four wild populations. Strain SPJ showed the lowest ge- netic diversity parameters values （Na=6.82, Ho=0.54 and PIC=0.50）, whereas Strain QSJ presented the highest parameters values （Na=21.25, Ho=0.80 and PIC=0.91）. The analysis of molecular variance indicates that most of the genetic variance was within popu- lations; however, significant genetic divergence among populations was detected （P〈0.01）. Similar results had been found by UP- GMA dendrogram and PCoA plots using Nei＇s genetic distances among six populations. The wild populations had closer genetic dis-tance and clustered together earlier, but were clearly separated from two selection strains. The PCoA plots and genetic structure ana- lysis reveal the distinct genetic structure of individuals from selection individuals and partial complexity genetic structure of wild individuals. In brief, wild C.carpio populations in China have high genetic diversity and good genetic purity in their selection strains.
作者
董在杰
刘念
傅建军
朱文彬
王兰梅
苏胜彦
DONG Zaijie;LIU Nian;FU Jianjun;ZHU Wenbin;WANG Lanmei;SU Shengyan(Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;College of Fisheries and Life Science,ShanghaiOcean University,Shanghai 201306,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2018年第4期46-55,共10页
South China Fisheries Science
基金
现代农业产业技术体系建设专项资金(CARS-45-05)
关键词
鲤
群体
选育
微卫星
遗传变异
Cyprinus carpio
population
selection
microsatellite
genetic diversity
