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煤气化废水降解菌的分离筛选与鉴定

Isolation Screening and Identification of Strains for Degradation of Coal Gasification Wastewater
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摘要 以苯酚为唯一碳源,从宁夏某煤气化工厂生化反应池活性污泥中分离筛选得到3株煤气化废水降解菌,分别命名为R、X和Y。考察发现3株菌株均对煤气化废水有降解作用,其中菌株R降解性能最优,在30℃、160 r/min下降解72 h,废水中化学需氧量(COD)和氨氮降解率分别为50%和46%。研究菌株R的降解性能与时间的关系,发现其对COD的降解在18 h后趋于稳定,对氨氮的降解在36 h后趋于稳定。对菌株R进行形态特征和分子生物学鉴定,结果显示菌株R与枯草芽孢杆菌的同源性达到99%。本研究筛选得到了具有应用潜力的优势菌株,为煤化工产业的污染治理问题提供了参考。 Three kinds of bacteria for coal gasification wastewater degradation,named R,X and Y,were isolated and screened from the activated sludge of a factory in Ningxia with phenol as the only carbon source.The results showed that these three strains could degrade the coal gasification wastewater with the best degradation performance over the strain R.The degradation rates of chemical oxygen demand(COD)andby the strain R reached 50%and 46%respectively after 72 h at 30℃and 160 r/min.The relationship between degradation rates and time over the strain R showed that the degradation rates of COD andtended to be stable after 18 h and 36 h,respectively.The morphological characteristics and molecular biological identification of the strain R showed that the homology between the strain R and bacillus subtilis reached 99%.This study screened out the superior strains with potential application,which provided a reference for the pollution control in the coal chemical industry.
作者 朱丽娜 张佑红 卢育兵 钱泽栋 ZHU Lina;ZHANG Youhong;LU Yubing;QIAN Zedong(School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,Chin)
出处 《武汉工程大学学报》 CAS 2018年第4期382-385,390,共5页 Journal of Wuhan Institute of Technology
关键词 煤气化废水 生物降解 分离筛选 coal gasification wastewater biodegradation strains isolation and screening
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