摘要
目的:利用顶空固相微萃取-气相色谱-质谱连用技术检测黄芪挥发油的化学成分,为黄芪的进一步开发和利用提供理论依据。方法:采用顶空固相微萃取技术对黄芪的挥发油成分进行富集,应用气相色谱-质谱联用法对检测出的化学成分进行分离和鉴定,采用面积归一法确定各成分的相对含量。结果:共检出挥发性成分65种,鉴定出其中的62个组分,占挥发油总量的97.70%。主要包括萜烯类和多环芳烃化合物;含量较高的分别是2-戊基呋喃(11.14%)、醋酸(10.69%)、姜黄烯(6.06%)、己腈(5.15%)、α-胡椒烯(3.91%)、椰子醛(3.69%)、羊油酸(3.48%)、正庚酸(3.26%)等。结论:黄芪挥发油化学成分丰富,具有很强的药用价值。顶空固相微萃取-气相色谱-质谱联用能全面快速的获得其组成信息,可应用于黄芪挥发油的快速分析。
Objective： To provide the theoretical basis for the further development and utilization of Astragalus membranaceus. The chemical constituents of volatile oil from Astragalus membranaceus were determined by solid phase microextraction-gas chromatography-mass spectrometry （SPME-GC-MS）. Methods： The essential oils were extracted by headspace solid phase micro-extraction （HS-SPME） then separated and identified by gas chromatography-mass spectrometry （GC- MS）. The relative content of each component was determined by area normalization. Results： Sixty-five compounds were detected from essential oils of tassel of carrot, and sixty-two of them were identified, which account for 97.70% of total flow area, mainly included terpene and polycyclic aromatic hydrocarbon compounds; the main constituents of the oil were 2-pentylfuran （11.14%）, Acetic acid （10.69%）, Curcumene （6.06%）, Hexanenitfile （5.15%）, a -Copaene （3.91%）, Prunolide （3.69%）, Caproic acid （3.48%）, oenanthic acid （3.26%）. Conclusion： There are abundant chemical constituents in the Volatile oil of Astragalus membranaceus, which has a strong medicinal value. SPME-GC-MS can get the information of its composition in a full and rapid way. It can be used for the rapid analysis of volatile oil from Astragalus membranaceus.
作者
杨娜
李英杰
周柏松
李雅萌
赵文杰
刘金平
李平亚
YANG Na;LI Ying-jie;ZHOU Bai-song;LI Ya-meng;ZHAO Wen-jie;LIU Jin-ping;LI Ping-ya(Jilin University,Ginseng Innovative Drug Development Local Joint National Engineering Research Center,Changchun 130021,China;jilin Jinbao Pharmaceutical Co.,Ltd,Baishan 135000,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第8期3627-3630,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
吉林省重大科技成果转化项目(No.20160301003YY)~~
