摘要
目的建立高效液相色谱法(HPLC)同时测定清热明目丸中绿原酸、柚皮苷、黄芩苷的含量。方法采用Agilent Eclipse XDB-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.4%磷酸溶液为流动相,梯度洗脱,流速为1.0 ml/min,检测波长为283 nm。结果绿原酸、柚皮苷、黄芩苷浓度分别在1.937~77.48μg/ml(r=0.9998)、1.028~41.12μg/ml(r=0.9999)、1.841~73.64μg/ml(r=0.9999)范围内与峰面积呈良好线性关系;平均加样回收率分别为98.6%,98.6%,99.1%,RSD分别为1.6%,1.2%,1.2%(n=9)。结论建立的分析方法能同时测定绿原酸、柚皮苷、黄芩苷3种成分,该方法快速简便、结果准确,可用于清热明目丸的质量控制。
Objective To establish an HPLC method for the simultaneous determination of chlorgenic acid, naringin and baicalin in Qingremingmu Pills. Methods An Agilent Eclipse XDB-C（18）（250 mm×4.6 mm, 5 μm） column was adopted. Gradient elution conducted, using acetonitrile-0.4 % phosphate solution as mobile phase with a flow rate of 1.0 ml/min. The detection wavelength was 283 nm. Results The concentrations of chlorogenic acid, naringin and baicalin showed good linear relationships with the corresponding peak areas in the range of 1.937-77.48 μg/ml（r=0.9998）, 1.028-41.12 μg/ml（r=0.9999） and 1.841-73.64 μg/ml（r=0.9999）, respectively. The average recoveries rates were 98.6 %, 98.6 % and 99.1 %, with RSD of 1.6 %, 1.2 % and 1.2 %（n=9）, respectively. Conclusion The methods can be applied to determination of chlorgenic acid, naringin and baicalin. It is simple and accurate, and can be used for the quality control of Qingremingmu Pills.
作者
丁晓爽
李惠
DING Xiao-shuang;LI hui(Zibo Food and Drug Inspection Institute,Zibo 255086,Chin)
出处
《食品与药品》
CAS
2018年第4期275-278,共4页
Food and Drug
