摘要
为探究蒙古栎天然次生林土壤微生物群落结构和多样性变化及其主要影响因素,采用Illumina Mi Seq高通量测序和OTU分析法比较辽东山区白石砬子自然保护区蒙古栎天然次生林(QMs)和辽宁省森林经营研究所实验林场蒙古栎天然次生林(QMp)土壤微生物细菌和真菌群落结构的差异,同时测定土壤理化性质,探究微生物群落结构与土壤环境因子的相关性。研究结果表明:QMs土壤的全碳、全氮含量分别为84.62g·kg^(-1)和7375.33mg·kg^(-1),都显著高于QMp,QMs土壤的pH值为4.89,显著低于QMp。QMs和QMp土壤细菌主要包括变形菌门、放线菌门、酸酐菌门、绿弯菌门、疣微菌门、芽单胞菌门、拟杆菌门、浮霉菌门和硝化螺旋菌门,其中,变形菌门为绝对优势种;真菌主要包括子囊菌门、担子菌门和接合菌门。QMp土壤细菌的Shannon指数为10.04,Simpson指数为0.9964;真菌的Shannon指数为7.30,Simpson指数为0.97,都显著高于QMs,ACE指数和Chao1指数都无显著差异。土壤pH值,全碳、全氮、速效氮含量是影响该区蒙古栎林土壤细菌群落结构差异的主要因素,而土壤pH值和全碳含量是影响该区蒙古栎天然次生林真菌群落结构差异的主控因子。
In order to explore the structure and diversity of soil microbial communities and its main influencing factors in Quercusmongolica natural secondary forests, we used the Illumina Mi Seqhigh throughput sequencing and OTU analysis to assess the composition and structure of bacteria and fungal community in Quercusmongolica natural secondary forests in the Baishilazi Nature Reserve（QMs） and the experimental forest farm of Liaoning Institute of Forest Management（QMp）. Meanwhile, in order to investigate the correlation between microbial community structure and soil environmental factors, the physicochemical properties of soil were also determined. The results showed that the contents of total carbon and total nitrogen in QMs were 84.62 g·kg-1 and 7375.33 mg·kg-1, respectively, which were significantly higher than that in QMp. However, the p H value in QMswas 4.89, which was significantly lower than QMp. The dominant bacterial groups were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi,Verrucomicrobia, Gemmatimonadetes, Bacteroidetes, Planctomycetes and Nitrospirae. The largest proportion of the bacteria community was Proteobacteria. The dominant fungal groups were Ascomycota, Basidiomycota and Zygomycota. The Shannon index and Simpson index of soil bacteria and fungiin QMp were 10.04, 0.9964, and 7.30, 0.97, respectively, which weresignificantly higher than that in QMs. And there were no significant in ACE index and Chao1 index between QMs and QMp. Soil p H, total carbon, total nitrogen and available nitrogen contents were the main factors affecting soil bacterial community structure and diversity of Quercusmongolica forest in this region. However, soil p H and total carbon were the main factors affecting soil fungal community structure and diversity of Quercusmongolica forest in this region.
作者
陈雅昕
邓娇娇
周永斌
殷有
魏亚伟
白雪娇
朱文旭
CHEN Ya-xin;DENG Jiao-jiao;ZHOU Yong-bin;YIN You;WEI Ya-wei;BAI Xue-jiao;ZHU Wen-xu(College of Forestry,Shenyang Agricultural University,Shenyang 110161,China;Research Station of Chinese Forest Ecosystem Research Network,Changtu Liaoning 112500,China)
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2018年第4期409-416,共8页
Journal of Shenyang Agricultural University
基金
国家重点研发计划项目子课题项目(2017YFC050410501)
辽宁省农业领域青年科技创新人才培养计划项目(2015047)
辽宁省博士启动基金项目(20170520064)
辽宁省宜林地营林决策平台构建及示范项目(201304216)
关键词
蒙古栎
天然次生林
高通量测序
微生物群落结构
Quercusmongolica
natural secondary forests
high-throughput sequencing
microbial community structure
